南海不同产地近江牡蛎中牛磺酸含量检测

doi:10.7506/spkx1002-6630-201310035

食品科学 ›› 2013, Vol. 34 ›› Issue (10): 164-168.doi: 10.7506/spkx1002-6630-201310035

南海不同产地近江牡蛎中牛磺酸含量检测

高加龙邱伟佳章超桦曹文红秦小明

1.水产品深加工广东普通高校重点实验室，广东湛江 524088；2.广东海洋大学食品科技学院，广东湛江 524088；
3.国家贝类加工技术研发分中心(湛江)，广东湛江 524088

收稿日期:2012-05-18 修回日期:2013-04-03 出版日期:2013-05-25 发布日期:2013-05-07
通讯作者: 章超桦 E-mail:zhangch2@139.com
基金资助:
国家现代农业产业技术体系

Determination of Taurine in Ostrea rivularis from Different Parts of the South China Sea

GAO Jia-long，ZHANG Chao-hua，QIU Wei-jia，CAO Wen-hong，QIN Xiao-ming

1. Key Laboratory of Aquatic Product Advanced Processing of Guangdong Higher Education Institutions, Zhanjiang 524088,
China；2. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China；3. The Sub Centre
(Zhanjiang) of National Technology and Research and Development of Shellfish Processing, Zhanjiang 524088, China

Received:2012-05-18 Revised:2013-04-03 Online:2013-05-25 Published:2013-05-07

摘要/Abstract

摘要：

建立牡蛎中牛磺酸含量检测的高效液相色谱方法。近江牡蛎样品经超声波处理，所得样液采用丹磺酰氯柱前衍生后C18色谱柱分离，紫外检测器定量检测，方法检出限1.2×10-5μg/mL，牛磺酸质量浓度在0.2～2.2μg/L范围内线性关系良好，R2为0.9932，方法加标回收率92.24%～96.73%。样液采用Amino-Na色谱柱分离，邻苯二甲醛(OPA)柱后衍生后荧光检测器检测，方法检出限0.022μg/mL，牛磺酸质量浓度在0.2～2.2μg/mL范围内线性关系良好，R2为0.9905，方法加标回收率为92.14%～96.73%。两种方法均具有较高的重现性和稳定性，OPA柱后衍生法精密度(RSD为0.81%)高于丹磺酰氯法(RSD为6.42%)。采用这两种方法分别检测南海不同产地近江牡蛎中的牛磺酸含量，结果无显著性差异，说明两种方法均适用于牡蛎等贝类样品中牛磺酸的检测。

关键词: 近江牡蛎, 牛磺酸, 高效液相色谱, 衍生

Abstract:

An HPLC method for the determination of taurine in Ostrea rivularis was established. The optimal conditions of
experimental parameters such as reaction temperature and mobile phase flow rate were established. The HPLC method was
validated with respect to accuracy, precision and limit of quantification. When Ostrea rivularis samples were extracted by a
ultrasonic-assisted extraction method, the resulting extract was separated on a C18 column following pre-column derivatization with
dansyl chloride, and quantitative detection was performed with a UV detector, the developed standard curve of taurine was linear
over the range of 0.2 to 2.2 μg/L, with correlation coefficient (R2) of 0.9932, the limit of detection (RSN = 3) was 1.2 × 10-5 μg/mL,
and the spike recoveries were between 92.24% and 96.73%, with relative standard deviation (RSD) of 6.42%. When the HPLC
method was based on separation on an Amino-Na column followed by post-column derivatization with orthophthalaldehyde
and fluorescence detection, the proposed standard curve of taurine was linear over range between 0.2 μg/mL and 2.2 μg/mL
(R2 = 0.9905), the limit of detection (RSN = 3) was 0.022 μg/mL, and the spike recoveries were between 92.14% and 96.73%,
with RSD of 0.81%. In conclusion, the two methods are simple, rapid, reliable and high accurate and precise and can be used to
determine taurine in Ostrea rivularis from different parts of the South China Sea with no significant differnce.

Key words: Ostrea rivularis, taurine, high performance liquid chromatography (HPLC), derivatization

中图分类号:

TS254.2

高加龙邱伟佳章超桦曹文红秦小明. 南海不同产地近江牡蛎中牛磺酸含量检测[J]. 食品科学, 2013, 34(10): 164-168.

GAO Jia-long，ZHANG Chao-hua，QIU Wei-jia，CAO Wen-hong，QIN Xiao-ming. Determination of Taurine in Ostrea rivularis from Different Parts of the South China Sea[J]. FOOD SCIENCE, 2013, 34(10): 164-168.

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南海不同产地近江牡蛎中牛磺酸含量检测

Determination of Taurine in Ostrea rivularis from Different Parts of the South China Sea

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