食品科学 ›› 2013, Vol. 34 ›› Issue (10): 204-206.doi: 10.7506/spkx1002-6630-201310044

• 分析检测 • 上一篇    下一篇

高效液相色谱测定益智仁中的牛磺酸含量

叶思平,陈悦娇,陈海光*,邓雅哲   

  1. 仲恺农业工程学院轻工食品学院,广东 广州 510225
  • 收稿日期:2012-03-18 修回日期:2013-04-09 出版日期:2013-05-25 发布日期:2013-05-07
  • 通讯作者: 叶思平 E-mail:691502091@qq.com

Determination of Taurine in Alpinia oxyphylla Miq. by High Performance Liquid Chromatography

YE Si-ping,CHEN Yue-jiao,CHEN Hai-guang*,DENG Ya-zhe   

  1. College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
  • Received:2012-03-18 Revised:2013-04-09 Online:2013-05-25 Published:2013-05-07

摘要:

建立高效液相色谱测定益智仁中牛磺酸含量的方法。经考察色谱流动相:甲醇-0.05mol/L的醋酸钠溶液(65:35,V/V),流速为1.0mL/min,DAD检测波长为UV-330nm。测定结果表明:样品中含有牛磺酸,线性范围为4~58mg/L,加标实验回收率为102.6%~105.3%,相对标准偏差为2.15%。益智仁中的牛磺酸的平均含量约为363.0mg/kg。

关键词: 益智仁, 高效液相色谱, 牛磺酸

Abstract:

A method to determine taurine in Alpinia oxyphylla Miq. by high performance liquid chromatography (HPLC) was
described. The mobile phase for chromatographic separation was made up of methanol and 0.05 mol/L sodium acetate (65:35,
V/V) at a flow rate of 1.0 mL/min. Detection wavelength was 330 nm. The results showed that dried fruits of Alpinia oxyphylla
Miq. were detected to contain taurine by the HPLC method. This method demonstrated a linear range of 4 to 58 mg/L. Average
recovery rates for taurine in spiked samples were between 102.6% and 105.3%, with RSD of 2.15%. The average taurine
content in dried fruits of Alpinia oxyphylla Miq. was 363.0 mg/kg.

Key words: Alpinia oxyphylla Miq., high performance liquid chromatography, taurine

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