榆干离褶伞溶栓酶对酒精诱导大鼠肝损伤的保护作用

doi:10.7506/spkx1002-6630-20200821-288

摘要/Abstract

摘要： 目的：研究榆干离褶伞溶栓酶（Lyophyllum ulmarium fibrinolytic enzyme，LUFE）对酒精诱导大鼠肝损伤的保护作用。方法：将大鼠随机分为4 组，即正常对照组，模型组，LUFE低、高剂量组。除正常对照组以外，其余各组每日按10 mL/kg mb灌胃体积分数40%的酒精诱导肝损伤。LUFE低、高剂量组分别以100、400 mg/kg mb的剂量灌胃LUFE，正常对照组和模型组以等量生理盐水灌胃，共28 d。末次给药后次日处死大鼠，苏木精-伊红染色观察大鼠肝组织病理形态学变化，检测血清丙氨酸氨基转移酶（alanine transaminase，ALT）、天冬氨酸氨基转移酶（aspartate aminotransferase，AST）、清蛋白（albumin，Alb）、γ-谷氨酰转移酶（γ-glutamyltranspeptidase，γ-GT）、碱性磷酸酶（alkaline phosphatase，ALP）、总胆红素（total bilirubin，T-BIL)、甘油三酯（triglyceride，TG）、总胆固醇（total cholesterol，T-CHO）、低密度脂蛋白胆固醇（low density lipoprotein cholesterol，LDL-C）和高密度脂蛋白胆固醇（high density lipoprotein cholesterol，HDL-C）水平。蛋白免疫印迹法检测抑制性-κBα（inhibitory kappa B-alpha，I-κBα）蛋白表达水平和核转录因子（nuclear factor，NF）-κB p65磷酸化（p-NF-κB）水平。结果：LUFE干预能减轻肝组织的病理性损伤，抑制酒精所致的血清AST、ALT、γ-GT、ALP活力及TG、T-CHO、LDL-C、T-BIL水平的升高和Alb水平的降低，其中高剂量LUFE能达到显著抑制效果（P＜0.05，P＜0.01）。Western blot结果表明，与模型组相比，LUFE干预能提高I-κBα蛋白表达水平，抑制NF-κB p65蛋白的磷酸化，其中高剂量LUFE能达到显著改善效果（P＜0.05，P＜0.01）。结论：LUFE对酒精所致大鼠肝损伤有保护作用，其机制可能与其抗氧化、抗炎作用有关。

关键词: 榆干离褶伞；溶栓酶；酒精；肝损伤

Abstract: Objective: To investigate the protective effects of fibrinolytic enzyme from Lyophyllum ulmarium (LUFE) on alcohol-induced liver injury in rats. Methods: Rats were randomly divided into four groups: normal control group, model group, and low- and high-dose LUFE groups. All rats except those in the normal control group were administered orally with 40% alcohol at a dose of 10 mL/kg mb body mass daily to induce liver injury. The animals in the low and high-dose LUFE groups were respectively treated with LUFE at doses of 100 and 400 mg/kg mb body mass, and those in the normal control group and the model group were given the same volume of normal saline by gavage for 28 days. The rats were sacrificed on the next day after the last administration, and the liver was dissected out to observe pathological morphological changes by hematoxylin-eosin staining and blood was withdrawn for detection of the levels of alanine transaminase (ALT) activity, aspartate aminotransferase (AST) activity, albumin (Alb) concentration, γ-glutamyltranspeptidase (γ-GT) activity, alkaline phosphatase (ALP) activity, total bilirubin (T-BIL) concentration, triglyceride (TG) concentration, total cholesterol (T-CHO) concentration, low density lipoprotein cholesterol (LDL-C) concentration and high density lipoprotein cholesterol (HDL-C) concentration. The expression level of inhibitory kappa B-alpha (I-κBα) protein and the phosphorylation level of nuclear factor (NF)-κB p65 were determined using Western blot assay. Results: LUFE alleviated liver the pathological damage, and inhibited the alcohol-induced increase in AST, ALT, γ-GT and ALP activities as well as TG, T-CHO, LDL-C and T-BIL levels and the alcohol-induced decrease in Alb level in the serum, with a significant inhibitory effect being observed at the high dose (P < 0.05 or 0.01). The Western blot results showed that LUFE increased the expression level of I-κBα protein and decreased the phosphorylation level of NF-κB p65 protein significantly at the dose (P < 0.05 or 0.01). Conclusion: LUFE has a protective effect on alcohol-induced liver injury in rats, and the underlying mechanism may be related to its antioxidant and anti-inflammatory effects.

Key words: Lyophyllum ulmarium; fibrinolytic enzyme; alcohol; liver injury

中图分类号:

R282.71

李芳芳，张蕊萌，丛贺，沈明花. 榆干离褶伞溶栓酶对酒精诱导大鼠肝损伤的保护作用[J]. 食品科学, 2021, 42(17): 121-126.

LI Fangfang, ZHANG Ruimeng, CONG He, SHEN Minghua. Protective Effects of Fibrinolytic Enzyme from Lyophyllum ulmarium on Alcohol-Induced Liver Injury in Rats[J]. FOOD SCIENCE, 2021, 42(17): 121-126.

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榆干离褶伞溶栓酶对酒精诱导大鼠肝损伤的保护作用

Protective Effects of Fibrinolytic Enzyme from Lyophyllum ulmarium on Alcohol-Induced Liver Injury in Rats

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