基于GC-MS分析不同品种小米代谢产物及代谢途径

doi:10.7506/spkx1002-6630-20210120-219

食品科学 ›› 2021, Vol. 42 ›› Issue (24): 199-205.doi: 10.7506/spkx1002-6630-20210120-219

• 成分分析 • 上一篇

基于GC-MS分析不同品种小米代谢产物及代谢途径

于润众，宗恩祥，张丽媛，张东杰

（1.黑龙江八一农垦大学信息与电气工程学院，黑龙江大庆 163319；2.黑龙江八一农垦大学食品学院，黑龙江大庆 163319；3.黑龙江省农产品加工与质量安全重点实验室，黑龙江大庆 163319；4.国家杂粮工程技术研究中心，黑龙江大庆 163319）

发布日期:2021-12-30
基金资助:
战略性国际科技创新合作重点专项（2018YFE0206300）；中央支持地方改革发展资金优秀青年人才项目（2020YQ16）；黑龙江省农垦总局重点研发项目（HKKY190407）；黑龙江省博士后科研启动项目（LBH-Q20165）

Analysis of Metabolites and Metabolic Pathways in Different Varieties of Foxtail Millets (Setaria italica L.) Based on Gas Chromatography-Mass Spectrometry

YU Runzhong, ZONG Enxiang, ZHANG Liyuan, ZHANG Dongjie

(1. College of Information and Electrical Engineering, Heilongjiang Bayi Agricultural University, Daqing 163319, China;2. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China;3. Key Laboratory of Agro-products Processing and Quality Safety of Heilongjiang Province, Daqing 163319, China;4. Chinese National Engineering Research Center, Daqing 163319, China)

Published:2021-12-30

摘要/Abstract

摘要： 基于气相色谱-质谱（gas chromatography-mass spectrometry，GC-MS）代谢组学方法研究同一产地不同品种小米的代谢产物。用甲醇溶液提取极性代谢组分，N,O-双(三甲基硅基)三氟乙酰胺衍生化处理，通过GC-MS分离鉴定代谢产物。结果表明，同一产地2?个不同品种小米共发现了53?种代谢产物。古龙小米有48?种代谢产物，其中优势代谢产物为亚油酸；禾绅小米代谢产物有32?种，优势代谢产物为亚油酸。对比发现，古龙小米有21?种差异代谢产物；禾绅小米有5?种差异代谢产物。对2?个品种小米差异代谢产物的代谢途径分析，发现古龙小米的葡萄糖代谢途径、脂肪酸代谢途径、氨基酸代谢途径和三羧酸循环都比禾绅小米对应的代谢途径更活跃，推测古龙小米的代谢产物多是因为其代谢速率快于禾绅小米的代谢速率。

关键词: 小米；代谢产物；气相色谱-质谱法；代谢组学；不同品种

Abstract: The metabolites in two different varieties of foxtail millets from the same geographical origin were studied by metabonomics based on gas chromatography-mass spectrometry (GC-MS). The polar metabolites were extracted using methanol solution and then derivatized with N,O-bis(trimethylsilyl)trifluoroacetamide prior to analysis by GC-MS. The results showed that a total of 53 metabolites were identified, 48 of which were found in ‘Gulong’ millet, the dominant ones being linoleic acid and 2-linolacyl-rac-glycerol; and 32 in ‘Heshen’ millet, the dominant ones being linoleic acid and glycerol. There were 21 differential metabolites identified in ‘Gulong’ millet and five differential metabolites in ‘Heshen’ millet. The metabolic pathways of the differential metabolites were also studied. It was found that the glucose, fatty acid, amino acid metabolism pathways and the tricarboxylic acid cycle in ‘Gulong’ millet were more active than those in Hesen millet. It was speculated that the increase in the number of metabolites in ‘Gulong’ millet may be because its metabolic rate was faster than that of ‘Heshen’ millet.

Key words: millet; metabolites; gas chromatography-mass spectrometry; metabonomics; different varieties

中图分类号:

O657.63

于润众，宗恩祥，张丽媛，张东杰,,. 基于GC-MS分析不同品种小米代谢产物及代谢途径[J]. 食品科学, 2021, 42(24): 199-205.

YU Runzhong, ZONG Enxiang, ZHANG Liyuan, ZHANG Dongjie. Analysis of Metabolites and Metabolic Pathways in Different Varieties of Foxtail Millets (Setaria italica L.) Based on Gas Chromatography-Mass Spectrometry[J]. FOOD SCIENCE, 2021, 42(24): 199-205.

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基于GC-MS分析不同品种小米代谢产物及代谢途径

Analysis of Metabolites and Metabolic Pathways in Different Varieties of Foxtail Millets (Setaria italica L.) Based on Gas Chromatography-Mass Spectrometry

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