食品科学 ›› 2025, Vol. 46 ›› Issue (20): 170-179.doi: 10.7506/spkx1002-6630-20250324-189

• 营养卫生 • 上一篇    下一篇

红曲黄色素纳米颗粒的制备及其对HepG2细胞脂质沉积的改善作用

林杨，孙思邈，信文娟，毛延卿，陈绕，邵平   

  1. （1.浙江工业大学食品科学与工程学院，浙江 杭州 310014；2.浙江佐力药业股份有限公司，浙江 湖州 313200；3.浙江养芝康生物科技有限公司，浙江 湖州 313200；4.杭州紫香糖业有限公司，浙江 杭州 311200；5.浙江工业大学莫干山研究院，浙江 湖州 313200）
  • 出版日期:2025-10-25 发布日期:2025-09-17
  • 基金资助:
    国家自然科学基金青年科学基金项目（32102006）；浙江省科技计划项目（2023C04007）

Preparation of Monascus Yellow Pigment Nanoparticles and Its Ameliorative Effect on Lipid Deposition in HepG2 Cells

LIN Yang, SUN Simiao, XIN Wenjuan, MAO Yanqing, CHEN Rao, SHAO Ping   

  1. (1. College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China; 2. Zhejiang Jolly Pharmaceutical Co., Ltd., Huzhou 313200, China; 3. Zhejiang Johncan Mushroom Biotechnology Co., Ltd., Huzhou 313200, China; 4. Hangzhou Zixiang Sugar Co., Ltd., Hangzhou 311200, China; 5. Moganshan Institute, Zhejiang University of Technology, Huzhou 313200, China)
  • Online:2025-10-25 Published:2025-09-17

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摘要： 为改善红曲米中红曲黄色素（Monascus yellow pigment，MYP）水溶性低和靶向性差的问题，提高其生物利用度，本实验从红曲米粉中提取MYP，在此基础上进一步制备了MYP-玉米醇溶蛋白-半乳糖基化壳聚糖（galactosylated chitosan，GC）纳米颗粒（MYP-GC-NP），并评估了其对油酸/棕榈酸诱导HepG2细胞脂质积累的改善作用。结果表明，MYP-GC-NP呈球形且均匀分布，其粒径为（167.00±0.60）nm，多分散性指数为0.25±0.01，ζ-电位为70.88 mV。此外，与未被半乳糖基化壳聚糖修饰的纳米颗粒相比，MYP-GC-NP更容易被肝细胞摄取，并能够显著减少细胞内脂质沉积。本研究可为改善MYP生物利用度提供新的思路，并为MYP的纳米颗粒在改善脂质沉积方面的应用提供理论依据。

关键词: 红曲黄色素；纳米颗粒；半乳糖基化壳聚糖；脂质沉积

Abstract: To address the poor water solubility and low targeting ability of Monascus yellow pigment (MYP) from Monascus rice and thereby enhance its bioavailability, this study prepared MYP-zein-galactosylated chitosan nanoparticles (MYP-GC-NP) and evaluated its efficacy in ameliorating oleic acid/palmitic acid (OA/PA)-induced lipid accumulation in HepG2 cells. The results showed that MYP-GC-NP exhibited a spherical shape and uniform size distribution with an average particle size of (167.00 ± 0.60) nm, a polydispersity index (PDI) of 0.25 ± 0.01, and a ζ-potential of 70.88 mV. Additionally, compared with nanoparticles without modification by GC, MYP-GC-NP were more readily taken up by hepatocytes and significantly reduced intracellular lipid accumulation. This study provides a novel strategy to improve the bioavailability of MYP and offers a theoretical basis for the application of MYP-loaded nanoparticles in alleviating lipid accumulation.

Key words: Monascus yellow pigment; nanoparticles; galactosylated chitosan; lipid deposition

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