白术多糖对局灶性脑缺血再灌注大鼠的神经保护作用

doi:10.7506/spkx1002-6630-200915052

食品科学 ›› 2009, Vol. 30 ›› Issue (15): 220-222.doi: 10.7506/spkx1002-6630-200915052

白术多糖对局灶性脑缺血再灌注大鼠的神经保护作用

王光伟¹,丰昀²,刘永乐³,李加兴⁴,邱细敏¹

1. 湖南师范大学医学院
2. 长沙市中心医院
3. 长沙理工大学生物与食品工程学院
4. 吉首大学食品科学研究所

收稿日期:2008-12-23 修回日期:2009-06-26 出版日期:2009-08-01 发布日期:2010-12-29
通讯作者: 王光伟 E-mail:wanggwwmq323@163.com
基金资助:
湖南省自然科学基金项目(08JJ3024)；长沙市科技局科技计划项目(K069062-12)

Neuroprotective Effect of Polysaccharide of Atractylodes macrocephala Koidz on Focal Cerebral Ischemia Reperfusion in Rats

WANG Guang-wei1，FENG Yun2，LIU Yong-le3，LI Jia-xing4，QIU Xi-min1

1. College of Medicine, Hunan Normal University, Changsha 410006, China；2. Changsha Central Hospital, Changsha 410004,
China；3. College of Biological and Food Engineering, Changsha University of Science and Technology, Changsha 410015,
China ；4. Institute of Food Science, Jishou Universtity, Jishou 416000, China

Received:2008-12-23 Revised:2009-06-26 Online:2009-08-01 Published:2010-12-29
Contact: WANG Guang-wei E-mail:wanggwwmq323@163.com

摘要/Abstract

摘要：

目的：探讨局灶性脑缺血再灌注后应用白术多糖治疗对神经功能的保护作用。方法：采用局灶性脑缺血再灌注模型，28 只雄性Sprague Dawley 大鼠随机分为对照组、再灌注组、蒸馏水组及白术多糖组。再灌注24h 进行神经功能缺损评分，取缺血区组织，分别检测其水分、Na+、K+ 含量，同时观察病理形态学改变情况。结果：白术多糖组神经功能缺损评分明显低于再灌注组及蒸馏水组(P＜0.05)；白术多糖组神经元损伤数量明显少于再灌注组及蒸馏水组(P ＜ 0.01)；白术多糖组水分、Na+(含量显著低于再灌注组及蒸馏水组(P ＜ 0.01)，K+ 含量显著高于再灌注组及蒸馏水组(P ＜ 0.01)。结论：白术多糖能减轻局灶性脑缺血再灌注后脑水肿的程度，减少神经细胞的受损，改善神经功能缺损状态，而起神经保护作用。

关键词: 白术多糖, 脑缺血, 再灌注, 神经保护

Abstract:

Objective: To study the neuroprotective effect of polysaccharide of Atractylodes macrocephala Koidz on focal cerebral ischemia reperfusion in rats. Methods: Rats were subjected to 1 h middle cerebral artery occlusion (MCAO) using an intraluminal nylon filament followed by 24 h reperfusion. Twenty-eight male Sprague Dawley rats were randomly divided into four groups: sham group, reperfusion group, distilled water group and polysaccharide of Atractylodes macrocephala Koidz group. The neurological deficit score was determined and the contents of water and total sodium, potassium were detected and pathological changes were observed in the ischemic brain tissues. Results and the neurological deficit scores in Atractylodes macrocephala Koidz group were significantly lower than those in reperfusion group and distilled water group (P＜0.05). The injured neurons in Atractylodes macrocephala Koidz group were significantly fewer than those in reperfusion group and distilled water group (P ＜0.01). The contents of water and total sodium in Polysaccharide of Atractylodes macrocephala Koidz group were significantly lower than those in reperfusion group and distilled water group (P＜0.01). The total potassium in polysaccharide of Atractylodes macrocephala Koidz group was significantly higher than that in reperfusion group and distilled water group (P ＜ 0.01). Conclusion: polysaccharide of Atractylodes macrocephala Koidz can show neuroprotective effect on focal cerebral ischemia reperfusion via alleviating brain edema, decreasing injured neurons and improving neurologic impairment.

Key words: polysaccharide of Atractylodes macrocephala Koidz, brain ischemia, reperfusion, neuroprotective effect

中图分类号:

Q946.3

王光伟1，丰昀2，刘永乐3，李加兴4，邱细敏1. 白术多糖对局灶性脑缺血再灌注大鼠的神经保护作用[J]. 食品科学, 2009, 30(15): 220-222.

WANG Guang-wei1，FENG Yun2，LIU Yong-le3，LI Jia-xing4，QIU Xi-min1. Neuroprotective Effect of Polysaccharide of Atractylodes macrocephala Koidz on Focal Cerebral Ischemia Reperfusion in Rats[J]. FOOD SCIENCE, 2009, 30(15): 220-222.

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白术多糖对局灶性脑缺血再灌注大鼠的神经保护作用

Neuroprotective Effect of Polysaccharide of Atractylodes macrocephala Koidz on Focal Cerebral Ischemia Reperfusion in Rats

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