食品科学 ›› 2009, Vol. 30 ›› Issue (16 ): 149-152.doi: 10.7506/spkx1002-6630-200916029

• 工艺技术 • 上一篇    下一篇

微波技术在鱼鳔蛋白提取中的应用

段振华1 ,2,孙小苓1,汪菊兰2   

  1. 1.海南大学食品学院 2.海南大学 热带生物资源教育部重点实验室
  • 收稿日期:2009-04-10 出版日期:2009-08-15 发布日期:2010-12-29
  • 通讯作者: 段振华 E-mail:dzh65@126.com
  • 基金资助:

    “十一五”国家科技支撑计划项目(2007BAD76B06);海南省自然科学基金项目(80409)

Application of Microwave Technology in Protein Extraction from Swim Bladder of Silver Carp

DUAN Zhen-hua1,2,SUN Xiao-ling1,WANG Ju-lan2   

  1. 1. College of Food Science and Technology, Hainan University, Haikou 570228, China;
    2. Key Laboratory of Tropic Biological Resources of Ministry of Education, Hainan University, Haikou 570228, China
  • Received:2009-04-10 Online:2009-08-15 Published:2010-12-29
  • Contact: DUAN Zhen-hua E-mail:dzh65@126.com

摘要:

选取新鲜鱼鳔为原料,以蛋白质提取率为指标,采用考马斯亮蓝法测定提取液中的蛋白质含量,分别研究固液比、微波萃取时间和微波功率等因素对鱼鳔蛋白提取的影响,并通过正交试验优化微波提取鱼鳔蛋白的工艺,得出在固液比1:30、萃取时间2.5min、萃取功率300W 条件下,鱼鳔蛋白质的提取率最高达22.79%。

关键词: 鱼鳔, 微波技术, 提取率, 蛋白质, 考马斯亮蓝

Abstract:

The microwave extraction of protein from swim bladders of silver carps was investigated. Four crucial extraction parameters were optimized to attain the highest extraction yield of protein by single-factor and orthogonal array designs. The optimal conditions for attain the maximum extraction yield of 22.79% were determined as follows: with the ratio of material to liquid (water) of 1:30, extraction at 300 W for 2.5 min. This study will provide a practical reference for the application of  microwave extraction in the preparation of protein from swim bladders.

Key words: swim bladder, microwave technology, extraction yield, protein, Coomassie brilliant blue

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