食品科学 ›› 2005, Vol. 26 ›› Issue (9): 506-508.

• 营养卫生 • 上一篇    下一篇

松针黄酮的体外抗氧化作用研究

 张培刚, 郑鸿雁, 昌友权, 曲红光   

  1. 吉林工程技术师范学院生物与食品工程系,吉林农业大学食品工程学院,吉林大学基础医学院药理教研室
  • 出版日期:2005-09-15 发布日期:2011-09-20

AntiOxidative Effect of Persimmon Leaf Flavonoid in vitro

 ZHANG  Pei-Gang, ZHENG  Hong-Yan, CHANG  You-Quan, QU  Hong-Guang   

  1. 1.Jilin Normal Institute of Engineering and Technology; 2.College of Food Engineering ,Jilin Agricultural University; 3.Department of Pharmacology, Basic School of Medical Sciences, Jilin University
  • Online:2005-09-15 Published:2011-09-20

摘要: 目的:研究松针黄酮的体外抗氧化作用。方法:用水杨酸法研究松针黄酮(pineneedieflavonoid,PF)清除•OH的效果,用硫代巴比妥酸法测定小鼠组织及肝线粒体、微粒体中丙二醛含量(MDA),用分光光度法测定小鼠红细胞溶血和肝线粒体膨胀程度研究PF的抗氧化效果。结果:PF可以清除•OH,抑制•OH所致丙二醛的产生,减少红细胞溶血,减轻肝线粒体膨胀程度。结论:可以认为PF具有明显的抗氧化作用。

关键词: 松针黄酮, 羟自由基, 抗氧化

Abstract: Objective: To study the anti-ox idative effects of PF. Methods: The•OH scavenging effect was measured by salicy lic acid method, the content of MDA in liver m itochondria and microsome was measured by TBA assay; hem olysis of RBC and the swelling of m itochondria were detected by spectrophotometric methods. Results: PF can scavenge•OH, inh ibit production of MDA, swelling of mitochondria and hem olys is of RBC. Conclusion: PF has anti-ox idation effects in vitro.

Key words: pineneedie flavonoid(PF), hydroxylradical, anti-oxidation