关键词: 枣, 高效液相色谱, 糖类

Abstract: The determination of carbohydrate in Chinese date was studied by means of High Performance Liquid Chromatog-raphy (HPLC). The jujube was dried, smashed first, and then extracted by ethanol. The extraction including the monosaccharideroligosaccharide, and polysaccharide which can be inputted directly to the HPLC. The remnant of extraction including thepolysaccharide was hydrolyzed, neutralized and concentrated. After constant volume, the hydrate was inputted into chromato-graphic column. The system of chromatography including the high-press constant-current pump was produced by DalianyiliteCompany, in China, and the refractive index detector, Waters 450. the chromatographic column HYDERSIR-NH2 were producedby Dalianyilite Company. The temperature in column and detector was 35℃. The flowing phase was CH3CN:CH3COOC2H5:H2O=60:25:15. The velocity of flow was 0.6ml/min. The input volume was 10μl. The result showed that the stability of galactose,fructose, rhamnose, xylose, arabinose, glucose, surose and mannose were 3.19%, 0.75%, 5.13%, 0.897%, 1.74%, 2.21%, 2.34%and 4.11% respectively. The coefficients of recovery of rhamnose, xylose, arabinose, fructose, mannose, glucose, galactose andsurose were 96.2%、99.3%、97.0%、110%、97.5%、97.0%、110%、110% respectively. The linear equation andcorrelation coefficient of rhamnose: Y=-3.87E-4+7.57E-5X and 0.995, of xylose: Y=1.40E-5+8.67E-5X, and 0.996, ofarabinose: Y=-1.24E-5+1.23E-4X, and 0.991, of fructose: Y=-2.78E-4+7.20E-5X, and 0.997, of mannose: Y=-1.98E-4+9.77E-5X, and 0.999, of glucose: Y=-1.75E-4+6.15 E-5X, and 0.999, of galactose: Y=1.91E-4+4.10E-5X, and 0.999, and of surose: Y=5.61E-5+6.60E-5X, and 0.999, respectively.

Key words: jujube, HPLC, carbohydrate