食品科学 ›› 2007, Vol. 28 ›› Issue (5): 358-362.

• 专题论述 • 上一篇    下一篇

酿酒酵母中β-葡聚糖生物合成的研究进展

 莫海珍, 刘晓永, 胡永金   

  1. 河南科技学院食品学院; 中国农业科学院农产品加工研究所; 云南农业大学食品学院 河南新乡453003; 北京100094; 云南昆明650201;
  • 出版日期:2007-05-15 发布日期:2011-12-31

Progress Review onβ-glucan Biosynthesis in Saccharomyces cerevisiae

 MO  Hai-Zhen, LIU  Xiao-Yong, HU  Yong-Jin   

  1. 1.Food College,Henan Institute of Science and Technology,Xinxiang 453003,China; 2.Institute of Processing for Agricultural Products,Chinese Academy of Agricultural Sciences,Beijing 100094,China; 3.School of Food Science,Yunnan Agricultural University,Kunming 650201,China
  • Online:2007-05-15 Published:2011-12-31

摘要: 本文详细地介绍了酵母β-葡聚糖(SCG)的生物合成的有关内容。酵母β-葡聚糖是以尿嘧啶二磷酸葡萄糖(UDP-Glc)为前体物质经葡聚糖合成酶(GS)将葡萄糖转移到葡聚糖主链的非还原端而生成可溶性的SCG,在壳聚糖合成酶Ⅲ(CSⅢ)作用下几丁质还原端以β-1,4键/β-1,2键的形式与β-1,3葡聚糖链支链的非还原端共价连接,形成了不可溶的SCG。此外,还对葡聚糖合成酶(GS)主要基因学的最新进展进行了阐述,其中FKS1和FKS2编码着β-1,3-葡聚糖酶的基本组分蛋白,RHO1基因则对β-1,3葡聚糖的合成进行调控;对β-1,6葡聚糖合成的基因尚不是很明确,目前已确定:KRE5、KRE6、CWH4p、ROT2和SKN1等基因对β-1,6葡聚糖的含量有着明显的影响。在此基础上,对有关酵母β-葡聚糖的将来研究方向提出了自己的见解。

关键词: &beta, -葡聚糖, 酿酒酵母, 合成, 基因

Abstract: Recent research developments ofβ-glucans biosynthesis in S.cerevisiae were detailedly reviewed in this paper.The biosynthesis of solubleβ-glucans resulted from the activity ofβ-glucans synthase and the sugar donor was uridine 5′- diphosphoglucose.The chitin synthascⅢwas capable of incorporating solubleβ-glucans and chitin viaβ-1,4 orβ-1,2 linkages, to changeβ-glucans from soluble to insoluble.Moreover,some current developments of related gene aboutβ-glucans synthase were summarized here.A pair of highly homologous proteins Fks lp and Fks 2p encoded by FKS1 and FKS2 genes,arc necessary inβ-glucans biosynthesis.The GTP-binding protein Rh1p is required for regulation ofβ-1,3-glucans biosynthesis.Many genes which contain KRES,KRE6,CWH4p,ROT2 and SKN1,arc known to affect the levels ofβ-1,6-glucans,but their role of the synthesis remains unknown.In the end,the future research direction and prospect ofβ-glucan’s biosynthesis were discussed.

Key words: &beta, -glucan; Saccharomyces.cerevisiae; biosynthesis; gene;