基于高效液相色谱法定量优化萹蓄黄酮提取工艺

食品科学 ›› 2012, Vol. 33 ›› Issue (14): 36-40.

基于高效液相色谱法定量优化萹蓄黄酮提取工艺

许福泉1，冯媛媛2，顾丽蕴1

1.淮海工学院江苏省海洋生物重点建设实验室 2.淮海工学院电子工程学院

收稿日期:2011-05-26 修回日期:2012-06-12 出版日期:2012-07-25 发布日期:2012-07-27
通讯作者: 许福泉 E-mail:sdfuquanxu@126.com
基金资助:
半叶马尾藻抗炎成分研究;海洋中药柽柳活性成分体内研究

Optimization of Extraction Process for Flavonoids from Polygonum aviculare L. Based on HPLC Quantitation

Received:2011-05-26 Revised:2012-06-12 Online:2012-07-25 Published:2012-07-27

摘要/Abstract

摘要： 目的：优化萹蓄黄酮提取工艺，提高提取液中目标黄酮成分含量。方法：利用高效液相方法考查正交试验在不同粒度、料液比、乙醇体积分数、提取时间、提取温度下目标黄酮总质量浓度，从而对提取工艺进行优化。结果：原料粒度对目标黄酮总质量浓度的影响最大，最佳提取工艺为粒度40目、料液比1:30(g/mL)、提取温度80℃、提取时间2h、乙醇体积分数65%，提取液中目标黄酮总质量浓度为228.9μg/mL。

关键词: 黄酮, 高效液相色谱, 提取工艺, 萹蓄

Abstract: Objective: To optimize the extraction condition of flavonoids from Polygonum aviculare L based on HPLC quantitation. Methods: The effects of raw material particle size, ethanol concentration, soaking (extraction) time, solid-to-solvent ratio and extraction temperature on the extraction rate of total flavonoids were explored by orthogonal array design. Results: The optimal extraction conditions were particle size of 40 mesh, solid-to-solvent ratio of 1:30 (g/mL), extraction temperature of 80 ℃, extraction time of 2 h and ethanol concentration of 65%. The total flavonoids concentration of the extract obtained under these conditions was 228.9μg/mL. Among all tested process parameters, raw material particle size had the biggest effect on the extraction rate of total flavonoids. Conclusion: HPLC provides a reliable method for quantifying total flavonoids. The optimize extraction process provides a reliable, simple and useful approach for the extraction and exploitation of flavonoids from Polygonum aviculare L.

Key words: flavonoid, high performance liquid chromatography (HPLC), extraction process, Polygonum aviculare L.

中图分类号:

TS201.1

许福泉1，冯媛媛2，顾丽蕴1. 基于高效液相色谱法定量优化萹蓄黄酮提取工艺[J]. 食品科学, 2012, 33(14): 36-40.

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