高效液相色谱法快速检测血管紧张素转换酶抑制活性

食品科学 ›› 2012, Vol. 33 ›› Issue (22): 196-199.

高效液相色谱法快速检测血管紧张素转换酶抑制活性

苑园园，崔同，苏旭东，马雯，张伟，檀建新*

河北农业大学食品科技学院，河北省农产品加工工程技术研究中心

收稿日期:2011-09-08 修回日期:2012-10-19 出版日期:2012-11-25 发布日期:2012-11-20
通讯作者: 苑园园 E-mail:yuanyuan827@yahoo.com.cn
基金资助:
国家海洋局贝类高值化利用技术中试研究与示范项目;微波作用下塑料包装材料化学物迁移动力学研究

Rapid HPLC Determination of Angiotensin Ⅰ-Converting Enzyme Inhibitory Activity

Received:2011-09-08 Revised:2012-10-19 Online:2012-11-25 Published:2012-11-20

摘要/Abstract

摘要： 建立高效液相色谱法快速检测血管紧张素转换酶(ACE)抑制活性的方法。色谱条件为：以ODS C18柱(150mm×4.6mm，5μm)为色谱柱，乙睛-水(20:80，V/V，含0.5‰甲酸)为流动相，流速1mL/min，检测波长为228nm。结果表明：马尿酸在0.1～500μg/mL质量浓度范围内线性关系良好，相关系数r=0.9999；平均回收率为89.77%～99.57%，RSD为0.01%～1.52%。不同的ACE抑制剂检测结果证明该方法操作简便快速、重现性好、准确度高，可以作为ACE抑制剂体外活性的检测方法。

关键词: 高效液相色谱, 血管紧张素转换酶, 马尿酸

Abstract: A rapid HPLC method was developed for the determination of angiotensin Ⅰ-converting enzyme (ACE) inhibitory activity. Hippuric acid was separated on an ODS C18 column (150 mm × 4.6 mm, 5 μm) using a mobile phase consisting of acetonitrile and water (20:80, V/V, containing 0.5‰ methanoic acid) at a flow rate of 1 mL/min and detected at 228 nm. A good linear relationship was observed for hippuric acid over the concentration range of 0.1 to 500 μg/mL with a correlation coefficient of 0.9999. The average spike recovery rates of hippuric acid were 89.77%–99.57% with a relative standard deviation (RSD) of 0.01%–1.52% (n = 3). The HPLC method was simple, rapid, reproducible, accurate and suitable for the analysis of ACE inhibitory activity in vitro as demonstrated by its applications to determine different ACE inhibitors.

Key words: high performance liquid chromatography (HPLC), angiotensin I-converting enzyme (ACE), hippuric acid (HA)

中图分类号:

TS207.3

苑园园，崔?同，苏旭东，马?雯，张?伟，檀建新*. 高效液相色谱法快速检测血管紧张素转换酶抑制活性[J]. 食品科学, 2012, 33(22): 196-199.

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