高效液相法测定成熟龙眼各部位游离鞣花酸含量

食品科学 ›› 2012, Vol. 33 ›› Issue (14): 181-183.

高效液相法测定成熟龙眼各部位游离鞣花酸含量

刘玉革，刘丽琴，刘胜辉，魏长宾

中国热带农业科学院南亚热带作物研究所

收稿日期:2011-07-04 修回日期:2012-06-11 出版日期:2012-07-25 发布日期:2012-07-27
通讯作者: 刘玉革 E-mail:liuyugehb@126.com

Determination of Free Ellagic Acid in Different Parts of Mature Longan by HPLC

Received:2011-07-04 Revised:2012-06-11 Online:2012-07-25 Published:2012-07-27

摘要/Abstract

摘要： 采用高效液相色谱法测定石峡、储量两个品种成熟龙眼各部位游离鞣花酸含量。鞣花酸提取条件为80%甲醇溶液，70℃回流2h。色谱条件为：C18色谱柱(250mm×4.5mm，5μm)、柱温35℃、进样体积10μL、检测波长254nm、流速1mL/min，流动相为甲醇-水-冰乙酸(50:49.7:0.3，V/V)。鞣花酸在1～200μg/mL范围内与峰面积呈线性关系，相关系数为0.999。取5个样品瓶分别进样，得到样品保留时间相对标准偏差(RSD)值为1.0%，峰面积RSD值为0.8%。对同一瓶样品连续进样10次，保留时间和峰面积的RSD值分别为0.15%和0.2%。加标回收结果表明平均加标回收率为95.97%～101.9%。测定结果表明石峡和储量各部位鞣花酸的含量大小顺序均为果核＞果皮＞果肉，其中果皮和果核中鞣花酸远大于已报道的其他水果中的含量。

关键词: 龙眼, 鞣花酸, 高效液相

Abstract: In this study, the contents of ellagic acid (EA) in different parts of mature longan cultivars Shixia and Chuliang were determined by HPLC. The sample extraction was achieved through hot reflux in 30 mL of 80% methanol aqueous solution for 2 h. The chromatographic separation was achieved on an Agilent C18 column (250 mm × 4.6 mm i.d., 5 μm) using a mobile phase composed of methanol, water and glacial acetic acid (50:49.7:0.3, V/V) at a flow rate of 1 mL/min. The injection volume, column temperature and detection wavelength were set at 10 μL, 35 ℃ and 254 nm, respectively. The linear range for EA was 1－200 μg/mL with a regression coefficient of 0.999. HPLC results showed that the content of EA in different parts of longan cultivars Shixia and Chuliang decreased in the following order: stone fruit ＞ peel ＞ pulp. The EA levels in the peel and pulp measured in this study were much higher than those reported in other fruits.

Key words: longan, ellagic acid, high performance liquid chromatography(HPLC)

中图分类号:

S667.4

刘玉革，刘丽琴，刘胜辉，魏长宾. 高效液相法测定成熟龙眼各部位游离鞣花酸含量[J]. 食品科学, 2012, 33(14): 181-183.

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