食品科学 ›› 2012, Vol. 33 ›› Issue (18): 191-194.

• 分析检测 • 上一篇    下一篇

高聚物萃取光度法测定水果硬糖中色素的含量

杜建中,董柳余,金 蓓   

  1. 湛江师范学院化学科学与技术学院
  • 收稿日期:2011-07-09 修回日期:2012-07-18 出版日期:2012-09-25 发布日期:2012-09-07
  • 通讯作者: 杜建中 E-mail:djz560119@126.com
  • 基金资助:

    广东省高校优秀青年创新人才培育项目

Determination of Pigments in Fruit Hard Candy by Polymer Extraction and Spectrophotometry

  • Received:2011-07-09 Revised:2012-07-18 Online:2012-09-25 Published:2012-09-07
  • Contact: Du Jian-zhong E-mail:djz560119@126.com

摘要: 利用水溶性高聚物溶液在无机盐存在条件下可分成两相的特点,将水果硬糖中的色素转入高聚物相,采用单波长法和双波长等吸收法对色素含量进行定量测定。结果显示,胭脂红在0.2~30μg/mL、柠檬黄在0.2~40μg/mL、诱惑红在0.2~35μg/mL、日落黄在0.2~30μg/mL、亮蓝在0.1~10μg/mL范围内有良好的线性关系,确定系数(R2)均大于0.997。该方法所需仪器设备简单,操作简单,准确度较高。

关键词: 聚乙二醇-4000, 水果硬糖, 分光光度法, 色素

Abstract: Based on the fact that aqueous solution of water-soluble polymers can be separated into two phases upon addition of inorganic salt, pigments in fruit hard candy were transferred into the polymer phase, resulting in reduced interference, and quantitatively determined by single-wavelength or dual-wavelength spectrometry. The results showed that the linear ranges of cochineal, lemon yellow, fancy red, sunset yellow, and bright blue were 0.2-30, 0.2-40, 0.2-35, 0.2-30 μg/mL and 0.1-10 μg/mL, respectively, with correlation coefficient (R2) of larger than 0.997. This method needs only simple equipment and is easy to use, and accurate.

Key words: polythene glycol-4000, fruit hard candy, spectrometry, pigment

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