茶树特异品种“紫娟”中的花青素组分分析

食品科学 ›› 2012, Vol. 33 ›› Issue (22): 203-206.

茶树特异品种“紫娟”中的花青素组分分析

吕海鹏1，费旭元1，梁名志2，王立波2，林智1,*

1.国家茶产业工程技术研究中心，中国农业科学院茶叶研究所 2.云南农业科学院茶叶研究所

收稿日期:2012-06-26 修回日期:2012-10-22 出版日期:2012-11-25 发布日期:2012-11-20
通讯作者: 吕海鹏 E-mail:lvhaipeng@caas.net.cn
基金资助:
现代农业产业技术体系建设专项资金;农业部茶与饮料植物产品加工与质量控制重点开放实验室开放基金

Analysis of Anthocyanins in Zijuan Tea

Received:2012-06-26 Revised:2012-10-22 Online:2012-11-25 Published:2012-11-20

摘要/Abstract

摘要： 目的：建立高效液相色谱法分析测定茶树特异品种“紫娟”的花青素组分及其含量。方法：采用高效液相色谱法梯度洗脱，YMC-Pack ODS-AQ C18色谱柱，流动相为体积分数0.5%甲酸溶液和乙腈，流速0.8mL/min，柱温35℃，检测波长520nm。结果：在选定的色谱条件下，天竺葵素-3,5-二葡萄糖苷(pelargonidin-3,5-diglucoside)、矢车菊-3-O-半乳糖苷(cyanidin-3-O-galactoside)、天竺葵色素(pelargonidin)和锦葵色素(malvidin)都得到较好分离；这4种花青素组分在0.03～0.10mg/mL的质量浓度范围内呈现良好的线性关系，相关系数均在0.999以上；精密度检测，RSD为0.73%～1.48%(n=5)；回收率在94.84%～96.46%之间；此外，测得“紫娟”烘青绿茶中上述4种花青素组分的含量分别为83.5、227.0、46.3μg/g和70.5μg/g。结论：该方法简单快捷、准确性和重复性好，可用于“紫娟”及其他茶叶中花青素组成的分析测定。

关键词: “紫娟”茶, 花青素, 组分, 高效液相色谱

Abstract: A high performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of four major anthocyanins in Zijuan tea, pelargonidin-3,5-diglucoside, cyanidin-3-O-galactoside, pelargonidin and malvidin. The chromatographic separation was achieved on a YMC-Pack ODS-AQ C18 column using a mobile phase made up of 0.5% formic acid and acetonitrile at a flow rate of 0.8 mL/min by gradient elution Column temperature and detection wavelength were set as 35℃ and 520 nm, respectively. The anthocyanins were separated well and showed good linear relationship over the concentration range of 0.03–0.10 mg/mL with correlation coefficients greater than 0.999. The precision RSDs of the HPLC method were 0.73%–1.48% (n = 5), and the recovery rates of these anthocyanins were in the range of 94.84%–96.46%. The contents of pelargonidin-3,5-diglucoside, cyanidin-3-O-galactoside, pelargonidin and malvidin in Zijuan tea were determined to be 83.5, 227.0, 46.3 μg/g and 70.5 μg/g, respectively. This method was convenient, rapid, accurate and applicable to determine anthocyanins in Zijuan tea and other tea samples.

Key words: Zijuan tea, anthocyanins, constituents, HPLC

中图分类号:

TS272

吕海鹏1，费旭元1，梁名志2，王立波2，林?智1,*. 茶树特异品种“紫娟”中的花青素组分分析[J]. 食品科学, 2012, 33(22): 203-206.

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