食品科学 ›› 2012, Vol. 33 ›› Issue (3): 101-104.doi: 10.7506/spkx1002-6630-201203022

• 生物工程 • 上一篇    下一篇

MTT法测定甲壳低聚糖对肠道微生物生长的影响

李 娜,肖凯军,庞 浩,王兆梅   

  1. 1.华南理工大学轻工与食品学院 2.中国科学院 广州化学研究所纤维素化学重点实验室
  • 出版日期:2012-02-15 发布日期:2012-02-14
  • 基金资助:
    国家自然科学基金项目(31071505);华南理工大学中央高校基本科研业务费专项(2009ZM0187); 华南理工大学广东省大学生创新实验项目(S1010561061)

Effect of Oligo-chitosan on the Growth of Intestinal Microorganisms as Determined by MTT Assay

LI Na,XIAO Kai-jun,PANG Hao,WANG Zhao-mei   

  1. 1. College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, China; 2. Key Laboratory of Cellulose and Lignocellulosics Chemistry, Guangzhou Institute of Chemistry, Chinese Academy of Sciences, Guangzhou 510650, China
  • Online:2012-02-15 Published:2012-02-14

摘要: 以肠道益生菌(保加利亚乳杆菌、嗜热链球菌)和肠道腐败菌(金黄色葡萄球菌、沙门氏菌)为研究对象,通过用MTT法来考察甲壳低聚糖对肠道微生物生长的影响。结果表明:在一定质量浓度(0.1~0.8g/100mL)范围内,甲壳低聚糖对肠道益生菌保加利亚乳杆菌、嗜热链球菌均有明显的增殖作用;随着菌体培养基糖(甲壳低聚糖)质量浓度的增大,甲壳低聚糖对肠道腐败菌金黄色葡萄球菌、沙门氏菌抑制效果增强;同时分子质量1kD的甲壳低聚糖比分子质量3kD的对微生物生长的影响更为显著。

关键词: MTT法, 甲壳低聚糖, 肠道微生物

Abstract: The effect of oligo-chitosan on the growth of intestinal microorganisms was studied using MTT assays. The results indicated that oligo-chitosan could obviously accelerate the proliferation of Lactobecillus bulgaricus and Streptococcu themophilus at the concentration range of 0.1-0.8 g/100 mL, while the antibacterial activity of oligo-chitosan against Staphylococcus aureus and Salmonella was strengthened with increasing oligo-chitosan concentration. The oligo-chitosan with molecular weight of 1 kD revealed more obvious impact on the growth of intestinal microorganisms than the oligo-chitosan with molecular weight of 3 kD.

Key words: MTT assay, oligo-chitosan, intestinal microorganisms

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