食品科学 ›› 2019, Vol. 40 ›› Issue (3): 217-223.doi: 10.7506/spkx1002-6630-20171207-090

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槐糖脂对蜜橘果实采后青霉病的控制及其机理

赵景芳1,2,何?银1,唐?杰1,龙晓琴1,曾凯芳1,曾凡坤1,*   

  1. (1.西南大学食品科学学院,重庆 400715;2.贵州省生物研究所,贵州?贵阳 550009)
  • 出版日期:2019-02-15 发布日期:2019-03-05
  • 基金资助:
    “十二五”国家科技支撑计划项目(2015BAD16B07)

Biocontrol of Postharvest Blue Mold (Penicillium italicum) on Satsuma Mandarin (Citrus unshiu Marc.) Fruit by Sophorolipid and Underlying Mechanism

ZHAO Jingfang1,2, HE Yin1, TANG Jie1, LONG Xiaoqin1, ZENG Kaifang1, ZENG Fankun1,*   

  1. (1. College of Food Science, Southwest University, Chongqing 400715, China; 2. Guizhou Institute of Biology, Guiyang 550009, China)
  • Online:2019-02-15 Published:2019-03-05

摘要: 从自然发病的蜜橘果实上分离出一株致病真菌,通过形态学特征、rDNA-内转录间隔区(rDNA-internal transcribed spacer,rDNA-ITS)分析以及致病性检测,发现该真菌为柑橘意大利青霉(Penicillium italicum)。离体实验发现,当槐糖脂质量浓度为0.1?g/L时,青霉的生长被完全抑制;在果实实验中,当槐糖脂质量浓度为35?g/L时,蜜桔果实的病斑直径最小,且发病率最低。采用质量浓度为35?g/L的槐糖脂对蜜桔果实进行接种和浸泡处理,均能有效提高蜜桔果实的抗病性,且蜜桔果皮的过氧化物酶、多酚氧化酶和苯丙氨酸解氨酶的活力得到了提高。该实验为蜜桔果实采后青霉病的防治提供了一定的理论依据。

关键词: 蜜桔, 青霉病, 槐糖脂, 诱导抗病性

Abstract: A pathogenic fungal strain was isolated from naturally infected satsuma mandarin fruit. On the basis of morphological characteristics, rDNA-internal transcribed spacer (rDNA-ITS) sequence analysis and pathogenicity test, the pathogen was identified as Penicillium italicum. The in vitro test showed that the growth of Penicillium italicum was completely inhibited at a sophorolipid concentration of 0.1 g/L; in the fruit test, the smallest lesion diameter and the lowest incidence of blue mold were observed at a sophorolipid concentration of 35 g/L. Treatment with 35 g/L sophorolipid effectively enhanced disease resistance in inoculated satsuma mandarin fruit by increasing the activities of peroxidase (POD), polyphenol oxidase (PPO) and phenylalanine ammonialyase (PAL). Our experimental results offer a theoretical basis for the prevention and control of postharvest blue mold in satsuma mandarin fruit.

Key words: satsuma mandarin, Penicillium italicum, sophorolipid, induced disease resistance

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