关键词: 黄酮, 超高效液相色谱, 体外模拟胃肠道消化, 生物可及性, 黄原胶

Abstract: Objective: Flavone C-glycosides?of bamboo leaves are susceptible to degradation during gastrointestinal digestion, leading to low bioaccessibility. Given this background, this study evaluated the changes in the bioaccessiblity of four flavone?C-glycosides (orientin, isoorientin, vitexin, and isovitexin)?present in the 80% ethanol extract of bamboo leaves at various stages of simulated gastrointestinal digestion in the presence of xanthan gum. Methods: Simulated digestion was carried out in the presence and absence of xanthan gum. The changes in flavone C-glycoside concentrations were analyzed by ultra-high performance liquid chromatography (UPLC). Results: Addition of xanthan gum significantly attenuated the degradation of flavone C-glycosides during the digestion process. The?oral, gastric and intestinal?phases had different impacts on the bioaccessibility of flavone C-glycosides. Xanthan gum could significantly enhance the bioaccessibility of the four flavonoid C-glycosides, especially vitexin and isovitexin. The concentrations of isovitexin and vitexin in the xanthan gum group decrease by 62% and 59% after intestinal digestion, respectively, which were much lower than those (decrease by 84% and 80%) observed in the presence of xanthan gum. Conclusion: Xanthan gum can significantly improve the bioaccessibility of bamboo leaf flavone?C-glycosides during simulated in vitro digestion, suggesting promising applications for the development of functional products.

Key words: flavonoids, ultra-high performance liquid chromatography, simulated in vitro gastrointestinal digestion, bioaccessibility, xanthan gum