Orthogonal Array Design as An Approach to Optimize Supercritical Fluid Extraction Conditions of Total Polyphenols from Punica granatum L. Leaves

doi:10.7506/spkx1002-6300-200918031

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (18 ): 152-154.doi: 10.7506/spkx1002-6300-200918031

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Orthogonal Array Design as An Approach to Optimize Supercritical Fluid Extraction Conditions of Total Polyphenols from Punica granatum L. Leaves

WANG Hua

Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400712, China

Received:2009-05-21 Online:2009-09-15 Published:2010-12-29
Contact: WANG Hua E-mail:wanghua40@126.com

Abstract

Abstract:

Total polyphenols in Punica granatum L. leaves were extracted using supercritical CO2 fluid with the addition of absolute ethanol as a cosolvent. In order to maximize yield of total polyphenols, four crucial extraction parameters such as ratio of material to liquid, and extraction pressure, temperature and time were optimized by single-factor and orthogonal array methods. A maximum yield of total polyphenols at 42.61% purity was obtained by the 120 min extraction at 25 MPa and 60 ℃ with a ratio of material to liquid of 1:2.5.

Key words: leaves of Punica granatum L., total polyphenols, supercritical fluid extraction

CLC Number:

WANG Hua. Orthogonal Array Design as An Approach to Optimize Supercritical Fluid Extraction Conditions of Total Polyphenols from Punica granatum L. Leaves[J]. FOOD SCIENCE, 2009, 30(18 ): 152-154.

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Orthogonal Array Design as An Approach to Optimize Supercritical Fluid Extraction Conditions of Total Polyphenols from Punica granatum L. Leaves

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