FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (5): 137-140.doi: 10.7506/spkx1002-6300-201005031

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Preliminary Study on Mechanism of GSE-induced Apoptosis in Saccharomyces cerevisiae

ZHANG Nan1,CAO Si-shuo2,HUANG Kun-lun1,2,LUO Yun-bo1,GUO Xing2,YUAN Yan-fang2,XU Wen-tao1,2,*   

  1. 1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;2. Supervision,
    Inspection and Testing Center of Genetically Modified Food Safety, Ministry of Agriculture, Beijing 100083, China
  • Received:2009-03-17 Online:2010-03-01 Published:2010-12-29
  • Contact: XU Wen-tao1,2,* E-mail:xuwentao1111@sina.com;cauxwt@yahoo.cn

Abstract:

In this study, grape seed extract (GSE) was used to induce apoptosis of Saccharomyces cerevisia and explore the mechanism of GSE-induced apoptosis. Results indicated that the minimum inhibition concentration of GSE on the growth of Saccharomyces cerevisiae was 0.13 mg/mL. Meanwhile, GSE resulted in nuclear condensation and DNA strand break of Saccharomyces cerevisiae cells according to TUNEL and DAPI staining. Detection of reactive oxygen species (ROS) also revealed that GSE could lead to a larger increase of ROS in Saccharomyces cerevisiae cells.

Key words: grape seed extract (GSE), Saccharomyces cerevisiae, mechanism of GSE-induced

CLC Number: