FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (4): 214-216.doi: 10.7506/spkx1002-6630-200904046

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Determination of Alliin in Garlic Using Precolumn Derivatization with AQC Followed by Reversed-phase High-performance Liquid Chromatography

YANG Yi,XIE Hui-ming,WU Ze-yu,WU Fang-rui   

  1. Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China
  • Received:2008-03-12 Revised:2008-09-26 Online:2009-02-15 Published:2010-12-29
  • Contact: YANG Yi E-mail:yangyi2900091@163.com

Abstract:

A rapid and reliable method for determination of alliin in garlic has been developed. Alliin in garlic was derivated by 6-aminoquinolyl-N-hydroxysuccinimicly carbamate in buffer at pH 8.5, and analyzed using HPLC equipped with Waters Symetry C18 column (150 mm × 3.9 mm, 5μm) and a fluorescence detector. The mobile phase was 0.02 mol/L potassium dihydrogen phosphate buffer at pH 5.5-acetonitrile (75:25, V/V) with the flow rate of 0.80 ml/min, and the excitation wavelength and emission wavelength were 250 nm and 395 nm, respectively. The results showed that there is a nice linear correlation between peak area and concentration of alliin in the range of 1 to 16 mg/L with the correlation coefficient of 0.99. The limit of detection is 1 mg/L, and the recovery is higher than 95%.

Key words: garlic, alliin, precolumn derivatization, high performance liquid chromatography

CLC Number: