Fractionation of Polysaccharides from Chicken Keel Cartilage and Their Free Radical Scavenging Activities

doi:10.7506/spkx1002-6630-200905014

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (5): 68-71.doi: 10.7506/spkx1002-6630-200905014

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Fractionation of Polysaccharides from Chicken Keel Cartilage and Their Free Radical Scavenging Activities

XIONG Shuang-li，ZHANG Jian-guo，YUAN Xiao-hong，WEI Ming

(School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China)

Received:2008-05-07 Revised:2008-06-24 Online:2009-03-01 Published:2010-12-29
Contact: XIONG Shuang-li E-mail:xiongshuangli@swust.edu.cn

Abstract

Abstract:

Crude polysaccharides were extracted from chicken keel cartilage by papain enzymolysis, deproteination of trichloracetic acid and ethanol precipitation, and then they were further purified by DEAE-Sepharose Fast Flow ion exchange column chromatography and Sepharose 6B Fast Flow gel column chromatography. Finally the scavenging activities of various fractions to hydroxyl radicals were compared. The results exhibited that the activity of crude polysaccharides precipitated by alcohol is significant higher than that of crude polysaccharides after preliminary dialysis. In addition, chondroitin sulfate and other 5 kinds of polysaccharides (non-glycosaminglycan) were obtained from the crude polysaccharides by DEAE-Sepharose Fast Flow ion exchange column chromatography and Sepharose 6B Fast Flow gel column chromatography. The scavenging activities of these 5 kinds of polysaccharides to hydroxyl radical are higher than that of chondroitin sulfate.

Key words: chicken keel cartilage, polysaccharide, fractionation, free radical

CLC Number:

Q514.3

XIONG Shuang-li，ZHANG Jian-guo，YUAN Xiao-hong，WEI Ming. Fractionation of Polysaccharides from Chicken Keel Cartilage and Their Free Radical Scavenging Activities[J]. FOOD SCIENCE, 2009, 30(5): 68-71.

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Fractionation of Polysaccharides from Chicken Keel Cartilage and Their Free Radical Scavenging Activities

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