Colorimetric Assay of Glycosylated Protein in Royal Jelly by Nitroblue Tetrazolium Chloride

doi:10.7506/spkx1002-6630-200924054

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (24 ): 256-259.doi: 10.7506/spkx1002-6630-200924054

Previous Articles Next Articles

Colorimetric Assay of Glycosylated Protein in Royal Jelly by Nitroblue Tetrazolium Chloride

GAO Tie-jun1,2，DONG Jie2，ZHANG Shou-wen1，TANG Feng-pei2，ZHANG Hong-cheng2,*

1. College of Food Engineering, Harbin University of Commerce, Harbin 150076, China；
2. Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China

Received:2009-08-01 Online:2009-12-15 Published:2010-12-29
Contact: ZHANG Hong-cheng2,* E-mail:zzhc@sohu.com

Abstract

Abstract:

In order to establish a rapid and convenient method for determining the content of glycosylated protein in royal jelly, a colorimetric assay was developed using nitroblue tetrazolium (NBT) chloride and 1-deoxy-1-morpholino-D-fructose (DMF) was used as the standard substance. Results indicated that the optimal reaction conditions between DMF and NBT were 57 ℃ of reaction temperature, 20 min of reaction time, 0.02% Triton, pH 10.8, and 0.5 mmol/L NBT. This method is a convenient and rapid determination method for glycosylated protein in royal jelly with high accuracy and sensitivity.

Key words: royal jelly, glycosylated protein, colorimetric assay

CLC Number:

TS201.2

GAO Tie-jun1,2，DONG Jie2，ZHANG Shou-wen1，TANG Feng-pei2，ZHANG Hong-cheng2,*. Colorimetric Assay of Glycosylated Protein in Royal Jelly by Nitroblue Tetrazolium Chloride[J]. FOOD SCIENCE, 2009, 30(24 ): 256-259 https://doi.org/10.7506/spkx1002-6630-200924054

[1]	WU Hongyan, QI Shanshan, ZHANG Lijun, HE Bo, ZHANG Yuxiu, NIE Xulian, JIANG Mengqiu, LI Zhiyuan, ZHENG Hongxing. Research Progress on Biological Activity of Royal Jelly and Its Application in Health Foods [J]. FOOD SCIENCE, 2025, 46(20): 406-420.
[2]	ZHU Wenzhen, CUI Can, ZHAO Xin, XUAN Hongzhuan. Research Progress on Protective Effect of Royal Jelly on the Cardiovascular System [J]. FOOD SCIENCE, 2025, 46(12): 347-356.
[3]	DU Juan, CHEN Xin, LIU Kai, LIU Jialei, LUO Pengjie, LIANG Dong, BAI Yanhong. Highly Sensitive Colorimetric Detection of Listeria monocytogenes by Using Gold Nanoparticles Based on Magnetic Metal-Organic Framework Separation [J]. FOOD SCIENCE, 2023, 44(6): 360-367.
[4]	ZHANG Wenwen, PANG Qian, WANG Kang, CHEN Xiaomei, FAN Rongli, CHEN Guohong, JI Ting. Effects of Different Dietary Sugars for Honeybees on Metabolite Composition in Royal Jelly Investigated by GC-QTOF-MS-Based Metabonomics [J]. FOOD SCIENCE, 2019, 40(4): 272-278.
[5]	WANG Xiaopeng, ZHAO Xinhuai. WANG Xiaopeng, ZHAO Xinhuai [J]. FOOD SCIENCE, 2019, 40(20): 47-53.
[6]	WANG Xueyu, QIAO Jiangtao, LIU Yinkang, ZHANG Hongcheng, DONG Jie. Separation of Major Royal Jelly Protein 1 Using Different Hydrophobic Chromatographic Columns [J]. FOOD SCIENCE, 2017, 38(1): 121-127.
[7]	ZHANG Zhongyin, ZHAO Liuwei, CAO Weirui, ZHOU Jinhui. Simultaneous Determination of Fluoroquinolone Residues in Honey and Royal Jelly by QuEChERS and High Performance Liquid Chromatography-Tandem Mass Spectrometry [J]. FOOD SCIENCE, 2016, 37(16): 242-248.
[8]	SHEN Fang, LIU Zhenguo, SHEN Jie, JI Ting. Effect of Royal Jelly on Growth and Development of Female Rats [J]. FOOD SCIENCE, 2015, 36(3): 202-206.
[9]	GUO Yahui, LIU Zhiyong, ZENG Zhijiang, CHEN Liling, ZHANG Jie, SHU Kun, ZHOU Linbin, PAN Qizhong. Protective Effect of Royal Jelly on Bromobenzene-Induced Oxidative Damage in Mice [J]. FOOD SCIENCE, 2015, 36(23): 253-256.
[10]	ZHANG Xin, ZHANG Hong-cheng, DONG Jie, ZHANG Gen-sheng. Purification and Circular Dichroism Analysis of MRJP1 Oligomer from Royal Jelly [J]. FOOD SCIENCE, 2014, 35(17): 63-67.
[11]	ZHAO Fang-yuan1,2，WU Ya-jun1，HAN Jian-xun1，CHEN Ying1,，GE Yi-qiang2,3,. A Two-dimensional Gel Electrophoresis Protocol for Proteomic Study of Royal Jelly [J]. FOOD SCIENCE, 2013, 34(9): 14-18.
[12]	. Separation of 10-Hydroxy-2-Decenoic Acid from Royal Jelly by Use of Macroporous Resin [J]. FOOD SCIENCE, 2013, 34(6): 116-119.
[13]	HU Yi-fan1,2，CHEN Zhong-zhou2，TIAN Wen-li1,2，ZHAO Ya-zhou1，PENG Wen-jun1,*. Circular Dichroism Analysis of MRJP2 Purified from Royal Jelly [J]. FOOD SCIENCE, 2013, 34(23): 57-61.
[14]	ZHOU Ping，LI Ying-hua，HU Fu-liang，ZHAO Huan，WU Jie，LIANG Shi-jun. Simultaneous Determination of Tau-Fluvalinate, Triadimenol and Coumaphos Residues in Royal Jelly Using High Performance Liquid Chromatography Tandem Mass Spectrometry [J]. FOOD SCIENCE, 2013, 34(18): 180-184.
[15]	GUO Zhan-bao，ZHAO Ya-zhou，WU Yu-xiang，LIU Jia-peng，PENG Wen-jun. Competitive Indirect ELISA for Determination of Streptomycin in Royal Jelly [J]. FOOD SCIENCE, 2013, 34(16): 206-209.

Colorimetric Assay of Glycosylated Protein in Royal Jelly by Nitroblue Tetrazolium Chloride

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments