Abstract:

In acidic condition, oxidation of ascorbic acid by Ce (IV) resulted in a poor chemiluminescence, and the addition of quinine exhibited a significant enhancement of luminescence. Furthermore, an excellent linear range between luminescence intensity and ascorbic acid concentration was observed. Thus, a flow injection chemiluminescence analytical method for determining ascorbic acid was developed. Under the optimal condition, the liner range for ascorbic acid was 0.03－1.0 mg/ml witha detection limit of 0.012 mg/ml. The relative standard deviation was 2.5% for 0.3 mg/ml ascorbic acid solution among 11 parallel determinations. Therefore, this proposed method can successfully used to determine quinine injection and tablets.

Key words: Ce (Ⅳ)-ascorbic acid-quinine, chemiluminescence, flow injection