Isolation, Purification and Antioxidant Activity of Polysaccharides from the Fruit Body of Panus giganteus (Berk.) Corner

doi:10.7506/spkx1002-6630-201014054

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (14): 238-240.doi: 10.7506/spkx1002-6630-201014054

• Analysis & Detection • Previous Articles Next Articles

Isolation, Purification and Antioxidant Activity of Polysaccharides from the Fruit Body of Panus giganteus (Berk.) Corner

FU Ming-hui，CHEN Jie-qiong

(Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China)

Received:2009-09-21 Revised:2010-04-25 Online:2010-07-15 Published:2010-12-29
Contact: FU Ming-hui E-mail:mhfugd@126.com

Abstract

Abstract:

The polysaccharides in the fruit body of Panus giganteus (Berk.) Corner were extracted with hot water, and precipitated with ethanol. The crude polysaccharides were then deproteinized by Sevage method, followed by DEAE 52 column chromatographic purification/fractionization. As a result, 2 fractions were obtained. Finally, fraction A, the first elution peak on DEAE 52 column was further purified by Sephadex S-200 column chromatography, and only one peak was observed in the elution profile, indicating this faction contains only a single component. It was found that fraction A could inhibit lipid peroxidation in mouse liver homogenate in vitro in a concentration-dependent way.

Key words: Panus giganteus (Berk.) Corner, polysaccharide, isolation and purification, antioxidant effect

CLC Number:

S646.19

FU Ming-hui，CHEN Jie-qiong. Isolation, Purification and Antioxidant Activity of Polysaccharides from the Fruit Body of Panus giganteus (Berk.) Corner[J]. FOOD SCIENCE, 2010, 31(14): 238-240.

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Isolation, Purification and Antioxidant Activity of Polysaccharides from the Fruit Body of Panus giganteus (Berk.) Corner

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