Screening and Identification of a Bacillus amyloliquefaciens Strain Producing Antimicrobial Substances

doi:10.7506/spkx1002-6630-201021047

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (21): 208-212.doi: 10.7506/spkx1002-6630-201021047

• Bioengineering • Previous Articles Next Articles

Screening and Identification of a Bacillus amyloliquefaciens Strain Producing Antimicrobial Substances

YANG Sheng-yuan1，WEI Jin2，LI Yun1，YAO Hong1，HUANG Yue-dan1

1. Department of Biology, Hanshan Normal University, Chaozhou 521041, China ；
2. Library of Hanshan Normal University, Chaozhou 521041, China

Received:2010-02-24 Online:2010-11-15 Published:2010-12-29
Contact: YANG Sheng-yuan E-mail:yshengyuan2004@yahoo.com.cn

Abstract

Abstract:

A strain producing broad-spectrum antimicrobial substances, named K6, was isolated and screened from the soil of a local vegetable garden in Xiangqiao district, Chaozhou city, which presented an inhibition effect on the growth of Mirococcus luteus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Bacillus thuringiensis, Saccharomyces cerevisiae, Rhizopus stolonifer and Candida albicans. The strongest inhibition effect on gram-positive bacteria was observed. Base on the morphological, 16S rDNA sequence and phylogenic analysis, the strain K6 was classified as Bacillus amyloliquefaciens.

Key words: antimicrobial substance, 16S rDNA, phylogenic analysis, Bacillus amyloliquefaciens

CLC Number:

Q939

YANG Sheng-yuan1，WEI Jin2，LI Yun1，YAO Hong1，HUANG Yue-dan1. Screening and Identification of a Bacillus amyloliquefaciens Strain Producing Antimicrobial Substances[J]. FOOD SCIENCE, 2010, 31(21): 208-212.

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Screening and Identification of a Bacillus amyloliquefaciens Strain Producing Antimicrobial Substances

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