Abstract:

In order to establish the fingerprint of HPLC analysis for tomentose pummelo (Exocarpium citri Grandis) peel, reversed-phase HPLC using Nucleosil C18 column (150 mm × 4.6 mm, 3μm) as the stationary phase, H2O-CH3OH-CH3CN as the mobile phase, triple gradient elution at the flow rate of 0.3－1 mL/min, DAD as the detector, column temperature at 35℃ and detection wavelength at 315 nm was used to determine the extract from tomentose pummelo peal. Results showed that 9 common peaks in HPLC chromatogram were observed in 11 samples. Through calculating by fingerprint analysis system of Chinese medicine, relative standard deviations (RSD) for relative retention time of common peaks were less than 0.3% and their similarity was more than 0.965. Therefore, a chromatographic fingerprint was established to provide scientific quality evaluation for tomentose pummelo peel.

Key words: HPLC, tomentose pummelo peel, coumarin, fingerprint