FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (2 ): 104-107.doi: 10.7506/spkx1002-6630-201102024

• Processing Technology • Previous Articles     Next Articles

Process Optimization for Total Polyphenol Extraction from the Tree Branch Bark of Xinjiang Black Mulberry (Morus nigra L.) by Response Surface Methodology

WU Chun1,XU Li1,*,LIU Jun-chi1,HUANG Xian-zhi1,MAIMAITI Yi-ming2   

  1. 1. College of Biotechnology, Southwest University, Chongqing 400716, China;
    2. Sericulture Research Institute of Hetian Xinjiang, Hetian 848000, China
  • Received:2010-03-15 Online:2011-01-25 Published:2011-01-10
  • Contact: XU Li E-mail:mulberry@swu.edu.cn

Abstract:

The objective of this study was the optimization of the extraction process for total polyphenols from the tree branch bark of Xinjiang black mulberry (Morus nigra L.) by response surface methodology. Based on single factor experiments, a threefactor, three-level Box-Behnken central composite design (CCD) coupled with response surface analysis (RSM) was employed to probe the effects of ethanol concentration, extraction temperature and length of extraction time on the yield of total polyphenols. It was found that the best extraction conditions were as follows: ethanol concentration, 78%; and liquid/material ratio, 30:1 (mL/g) for an extraction duration of 240 min at 70 ℃, and that a maximum total polyphenol yield of 0.793% under such conditions. The extraction process proves stable and reliable and is applicable for the production of polyphenols.

Key words: Morus nigra L., bark mulberry tree bark, total polyphenols, response surface methodology

CLC Number: