FOOD SCIENCE ›› 2014, Vol. 35 ›› Issue (2): 56-61.doi: 10.7506/spkx1002-6630-201402011

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Optimization of Extraction Conditions for Collagen from Zhedong White Goose Skin by Response Surface Methodology

DING Lin, ZHANG Yu-lin, YANG Yuan, ZHAO Zi-wei, PAN Dao-dong*   

  1. School of Marine Sciences, Ningbo University, Ningbo 315211, China
  • Received:2013-03-06 Revised:2013-12-31 Online:2014-01-25 Published:2014-02-19
  • Contact: PAN Dao-Dong E-mail:daodongpan@163.com

Abstract:

The objective of this study was to optimize the acidic extraction of collagen from Zhedong white goose (a
famous local breed of Chinese geese for meat production) skin. Acid type, pH, solid-to-liquid ratio and extraction time were
considered in this study. Lactic acid was selected as optimal extraction solvent. One-factor-at-a-time method and response
surface methodology were designed for the optimization of extraction conditions. The maximum extraction yield of collagen
was experimentally observed as 30.12% under the optimized conditions: pH 1.9, solid-to-liquid ratio (g/mL) of 1:50 and
extraction time of 60 h, showing a relative deviation of 0.13% compared with the predicted value. The sample obtained was
identified as type Ⅰ collagen by ultraviolet spectroscopy. Meanwhile, intact triple helix structure was also identified by
infrared spectroscopy.

Key words: goose skin, collagen, lactic acid, response surface methodology

CLC Number: