Abstract:

Objective：To explore the effect of nonylphenol (NP) on inducing injury and changing ROS and GSH contents in
NCTC1469 cells. Methods: Cultured cells were treated with NP (0, 0.01, 0.1, 1, 10 μmol/L and 100 μmol/L) for 24 h. Then,
cell viability was assessed with a cell counting kit; the NP-induced injury was evaluated by detecting lactate dehydrogenase
(LDH), alanine aminotransamine (ALT) and aspartate aminotransaminase (AST) activities in cell culture supernatant and
the contents of intracellular glutathione (GSH) and reactive oxygen species (ROS) were assayed by flow cytometric method.
Results: NP could inhibit NCTC1469 cell proliferation and promote ROS generation significantly in a dose-dependent
manner. Compared with the control group, the LDH and ALT activities in cell culture supernatant from 0.1–100 μmol/L
NP groups had a significant increase, while the AST activity increased dramatically only in 1–100 μmol/L NP groups. NP
could decrease intracellular GSH content in a dose-dependent manner, and there was a significant difference when compared
with the control group. Conclusion: NP can induce NCTC1469 cells injury by up-regulating the level of ROS and downregulating
the content of GSH.

Key words: nonylphenol, NCTC1469 cells, oxidative stress