Determination of Pantothenic Acid in Formula Milk Powder Using Ultra Performance Liquid Chromatography-Isotope Dilution Mass Spectrometry

doi:10.7506/spkx1002-6630-201408042

Abstract

Abstract:

An ultra performance liquid chromatography-isotope dilution mass spectrometry (UPLC-IDMS) method has been
developed for the determination for pantothenic acid (PA) in formula milk powder. Samples were extracted with ammonium
acetate solution, and precipitated protein by adding chloroform for analyzing by UPLC-MS-MS. The analyte was separated
using an HSS T3 column. The mobile phase consisted of 10 mmol/L ammonium acetate with 0.1% formic acid and
acetonitrile. The PA was identified by multiple reaction monitoring (MRM) and quantified by internal standard method.
The results showed that the limit of quantification for PA was 0.040 mg/100 g. The recoveries at three spiked levels and the
relative standard deviations were 96.9%–104.3% and 3.78%–5.04%, respectively. This method proved to be simple, timesaving,
sensitive and accurate for the determination of PA in formula milk powder.

Key words: pantothenic acid, ultra performance liquid chromatography-isotope dilution mass spectrometry, formula milk powder

CLC Number:

QU Yan1, CUI Ya-juan1, LI Quan-xia1, CHEN Zhao-tian1, LI Dong1, LIANG Min-hui2. Determination of Pantothenic Acid in Formula Milk Powder Using Ultra Performance Liquid Chromatography-Isotope Dilution Mass Spectrometry[J]. FOOD SCIENCE, 2014, 35(8): 212-216.

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