Chemical Structure Analysis of Sialyglycoproteins of Crucian Carp Carassius auratus Eggs and the Effect of the Sialyglycoproteins on Proliferation and Differentiation of MC3T3-E1 Cells

doi:10.7506/spkx1002-6630-201413039

Abstract

Abstract:

This study was designed to investigate the chemical structure of sialyglycoproteins from crucian carp Carassius
auratus eggs (CA-SGP) and the effects of CA-SGP on the proliferation, differentiation and mineralization of MC3T3-E1
cells. CA-SGP was isolated from the water soluble protein of crucian carp eggs using anion exchange chromatography and
gel exclusion chromatography, and its composition was determined. The proliferation of MC3T3-E1 cells was determined
by MTT assay, the secretion of collagen type (COLⅠ), alkaline phosphatase (ALP), and osteocalcin (OCN) were detected
by enzyme-linked immunosorbent assay (ELISA) and the mineralized nodule of MC3T3-E1 cells was evaluated by staining
with Alizarin red S dye. The analysis of chemical structure showed that the CA-SGP was composed of 14.33% protein
and 62.81% carbohydrates in which the content of N-acetylneurainic acid (Neu5Ac) was 19.72%, and the monosaccharide
composition of CA-SGP was Man, GlcN, GalN, and Neu5Ac. The activity assays indicated that the CA-SGP effectively
promoted the proliferation of MC3T3-E1 cells, significantly increased the secretion of ALP, OCN and COLⅠand enhanced
the mineralization ability. In conclusion, CA-SGP significantly promotes the proliferation, differentiation and mineralization
of MC3T3-E1 cells and can be used as an active ingredient in a safe and effective anti-osteoporosis functional food.

Key words: sialyglycoproteins, isolation and purification, MC3T3-E1 cell, proliferation, differentiation

XIA Guang-hua, HE Min, ZHAN Qi-ping, XUE Chang-hu, WANG Jing-feng. Chemical Structure Analysis of Sialyglycoproteins of Crucian Carp Carassius auratus Eggs and the Effect of the Sialyglycoproteins on Proliferation and Differentiation of MC3T3-E1 Cells[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201413039.

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Chemical Structure Analysis of Sialyglycoproteins of Crucian Carp Carassius auratus Eggs and the Effect of the Sialyglycoproteins on Proliferation and Differentiation of MC3T3-E1 Cells

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