Abstract:

Using high-pressure homogenization, we have examined the effects of different homogenization conditions
such as emulsifier type and concentration, temperature and co-emulsifier on the physicochemical properties of fucoxanthin
nanoemulsion (FCNE). The results showed that the droplet diameter decreased with increasing homogenization pressure
and number. The minimum droplet diameter (154 nm, PDI = 0.19) could be produced after 4 consecutive treatments at 120
MPa. The droplet diameter also depended strongly on emulsifier type: SDS < Tween 80 < sodium caseinate, and decreased
with emulsifier concentration and holding temperature (P < 0.05). For Tween 80 and SDS stabilized FCNEs, the droplet size
decreased appreciably with glycerol concentration, while changed little for the sodium caseinate stabilized one, indicating
that Tween 80 and SDS stabilized FCNEs were more sensitive to viscosity than sodium caseinate stabilized FCNE. The
electrical charge and droplet diameter were constant at different pH conditions for Tween 80 stabilized FCNE, while SDS
and sodium caseinate stabilized ones underwent great changes both in charge and diameter at acid conditions (P < 0.05).
After storage at 37 ℃ for 30 days, the amount of residual fucoxanthin was higher in FCNE than in organic phase, suggesting
FCNE could enhance the stability of fucoxanthin obviously.

Key words: fucoxanthin, nanoemulsion, high-pressure microfluidization, factor