Abstract:

Squalene certified reference material was developed by purification of crude squalene. The structure of the
certified reference material was confirmed by infrared spectroscopy (IR), high resolution mass spectrometry (HRMS) and
nuclear magnetic resonance (NRM) and squalene was divided into 150 aliquots. The homogeneity and stability tests and
quantitative analysis were carried out by gas chromatography-flame ionization detection (GC-FID). Fifteen randomly
selected aliquots showed good homogeneity as indicated by F-test analysis at a 95% confidence interval. The test samples
were stable at −18 ℃ for 24 months. A cooperative certification was conducted with 8 qualified laboratories. The certified
purity value of the reference material was 99.57% with a relatively expanded uncertainty of 0.30% (k = 1.96). The reference
material can conform to the technical requirement of the certified reference material. This material was intended for use in
the method validation and quality control regarding squalene.

Key words: squalene, certified reference material, gas chromatography-flame ionization detection (GC-FID), certification, uncertainty