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In Vitro and in Vivo Anti-Hyperglycemic Effects of Polysaccharides from Auricularia auricular

YIN Hongli1,2, ZHAO Xin1, TONG Lili3, WANG Zhenyu4   

  1. 1. School of Forestry, Northeast Forestry University, Harbin 150040, China; 2. Institute of Staple Food Processing Technology,
    Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Harbin 151900, China;
    3. Hongqi Hospital of Mudanjiang Medical University, Mudanjiang 157011, China;
    4. School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin 150090, China
  • Online:2015-11-15 Published:2015-12-03

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Abstract:

Objective: To explore the hypoglycemic effects of Auricularia auricular polysaccharides in vitro and in
vivo. Methods: The inhibitory effects of acidic, neutral and alkaline polysaccharides from Auricularia auricular as
well as ethanol-precipitated fractions of acidic polysaccharides on α-glucosidase activity were investigated by in vitro
microwell plate-based screening method. The purified polysaccharide was used to treat rat models of diabetes induced by
alloxan. The changes in body weight, fasting blood glucose (FBG), hexokinase (HK), and succinic dehydrogenase (SDH)
were determined in diabetic rats before and after treatment. Results: Based on the hypoglycemic effect in vitro, Auricularia
auricular polysaccharides had inhibitory effect on α-glucosidase. Auricularia auricular acidic polysaccharides had the
strongest inhibitory activity against α-glucosidase followed by the neutral polysaccharides, and the alkaline polysaccharides
were the weakest α-glucosidase inhibitor. The inhibitory activity of 80% alcohol-precipitated fraction was stronger than
that of other alcohol concentrations. According to in vivo hypoglycemic results, acidic polysaccharides from 80% alcohol
precipitation fraction could significantly attenuate the negative growth of body weights in diabetic rats, and significantly
increase HK and SDH. Therefore, Auricularia auricular acidic polysaccharides have significant hypoglycemic effect.

Key words: Auricularia auricular polysaccharides, Auricularia auricular acidic polysaccharides, α-glucosidase, diabetes

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