Abstract:

Objective: To explore the therapeutic effect of burdock fructooligosaccharide (BFO) combined with Lactobacillus
plantarum Sc52 on mice with type 2 diabetes induced by high-fat diet and streptozocin (STZ). Methods: C57BL/6J mice
were intraperitoneally injected with STZ and fed with high-fat diet to establish a mouse model of type 2 diabetes. Then,
Sc52, BFO and Sc52 combined with BFO were respectively given to mice by gavage for 4 consecutive weeks. The
fasting blood glucose and tolerance dose were measured by Glucose Test Strips, serum lipids, INS, inflammatory factors
and other biochemical indicators were also tested by kits, the contents of short-chain fatty acids were determined by gas
chromatography and the major intestinal bacteria in feces were counted by coated plates. Results: L. plantarum Sc52 and
BFO alone or in combination could inhibit the elevation of TC, TG, LDL-C, ET, TNF-α and IL-6 in serum and significantly
increase HDL-C and INS levels in serum. The contents of acetic acid, propionic acid and butyric acid in intestine were
raised but the content of lactic acid was decreased. L. plantarum Sc52 combined with BFO could promote the growth of
Lactobacillus and Bifidobacterium, and inhibit the growth of Enterobacilli, Enterococcus, Clostridium perfringens and other
harmful bacteria in the intestine of mice. The therapeutic effect of L. plantarum Sc52 combined with BFO was better than that
either of them alone. Conclusion: L. plantarum Sc52 combined with BFO may improve insulin in a mouse model of type 2
diabetes by regulating the balance of intestinal flora in mice, decreasing the concentrations of blood glucose and serum lipids
and inhibiting inflammatory cytokines.

Key words: Lactobacillus plantarum, burdock fructooligosaccharide, type 2 diabetes, intestinal microflora, obesity