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Optimization of Extraction Conditions of Crude Polysaccharide from Auricularia auricular and Its Effects on the Functions of Mouse Peritoneal Macrophages

XU Hailin1, WU Xiaoyong2,*, NIE Shaoping3, HUANG Yansheng4, TANG Qingtao4   

  1. 1. School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China; 2. School of Food Science,
    Guangdong Pharmaceutical University, Zhongshan 528453, China; 3. State Key Laboratory of Food Science and Technology,
    Nanchang University, Nanchang 330047, China; 4. Infinitus (China) Co. Ltd., Xinhui 529156, China
  • Online:2016-05-25 Published:2016-05-18

Abstract:

In this study, the extraction conditions of water-soluble polysaccharide from dried fruiting bodies of Auricularia
auricular were investigated by single-factor and orthogonal array design methods. The influence of liquid-to-solid ratio,
stirring speed, extraction time, extraction temperature and the particle size of the dried material on extraction efficiency
was analyzed. The optimal extraction conditions were established based on the extraction yield of polysaccharide. Results
showed that the most important factor was the particle size of the dried material, followed by extraction time, stirring speed
and liquid-to-solid ratio. The optimal parameters determined in this study were as follows: the particle size, 80 mesh; liquidto-
solid ratio, 30:1(mL/g); stirring speed, 200 r/min, extraction temperature, 100 ℃; and extraction time, 70 min. Under
these conditions, the polysaccharide yield from A. auricular was 8.46%. The effects of the crude polysaccharide extracted
from A. auricular on the immunologic functions of mouse macrophages were also investigated through carbon clearance
test and peritoneal macrophage phagocytosis function test using fluorescence labeled microspheres. Additionally, the effects
of the crude polysaccharide on immune organs were also examined. Results indicated that while the crude polysaccharide
had no significant influences on the body weight, thymus index or spleen index of the animals, it significantly improved the
phagocytosis of mononuclear macrophage. In conclusion, the crude polysaccharide extracted from A. auricular can enhance
animal nonspecific immunity.

Key words: Auricularia auricular, polysaccharide, extraction process, macrophage

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