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Comparative Detection of Lactose and Sucrose in Milk Powder by Lane-Eynon’s Method and High Performance Liquid Chromatography with Refractive Index Detector

GUO Xiuchun, GUO Xiaobai, ZHANG Miaomiao, MA Jiaohao, KANG Wenyi   

  1. 1. Institute of Chinese Materia, Pharmaceutical College, Henan University, Kaifeng 475004, China;
    2. Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, China;
    3. Minsheng College, Henan University, Kaifeng 475004, China
  • Online:2016-06-25 Published:2016-06-29
  • Contact: KANG Wenyi E-mail:kangweny@hotmail.com

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Abstract:

Lactose and sucrose in milk powder from the same commercial brand for two different age groups were assayedby the Lane-Eynon’s method and high performance liquid chromatography with refractive index detector (HPLC-RID). The differences in lactose and sucrose contents among different samples were analyzed, and the results of the two methods were compared. It was indicated that the accuracy of the HPLC-RID method was higher than that of the Lane-Eynon’s method. The linear ranges of the HPLC method for sucrose and lactose were both 2–12 mg/mL with correlation coefficients of 0.999 3 and 0.999 6, respectively. The precision, expressed as relative standard deviations, for sucrose and lactose were 2.53% and3.81%, respectively. The average recovery rates were 99.70% and 101.06%, respectively. The HPLC method had some advantages such as high accuracy, short analysis time, simple sample pretreatment and suitability for rapid determination of sucrose and lactose contents in milk powder.

Key words: Lane-Eynon’s method, high performance liquid chromatography with refractive index detector (HPLC-RID);
milk powder, lactose, sucrose

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