Optimization of Sulfation Conditions and Infrared Spectroscopy Analysis of Nostoc flagelliforme Exopolydsaccharide

doi:10.7506/spkx1002-6630-201624009

Abstract

Abstract: A purified exopolydsaccharide was obtained from the cell-free culture broth of Nostoc flagelliforme cells by concentration, dialysis, deproteinization, alcohol precipitation and sequential column chromatographies on DEAE-52 cellulose and Sephadex-100. By combined use of one-factor-at-a-time method and Box-Behnken response surface methodology, the optimum sulfation conditions for maximum degree of substitution (DS) of sulfate groups in the polydsaccharide (5.05) were obtained as follows: reaction temperature, 71 ℃; reaction time, 3 h; and chlorosulfonic acid-pyridine ratio, 1:7 (V/V). The infrared spectroscopy analysis showed the binding of sulfuric acid groups to the polysaccharide molecule.

Key words: Nostoc flagelliforme, polysaccharide, sulfation, infrared spectroscopy

CLC Number:

TS201.2

GUO Jinying, ZHU Beiru, REN Guoyan, WU Ying, WANG Ping, CUI Guoting, LI Zhengjun. Optimization of Sulfation Conditions and Infrared Spectroscopy Analysis of Nostoc flagelliforme Exopolydsaccharide[J]. FOOD SCIENCE, 2016, 37(24): 61-67.

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Optimization of Sulfation Conditions and Infrared Spectroscopy Analysis of Nostoc flagelliforme Exopolydsaccharide

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