FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (9): 99-106.doi: 10.7506/spkx1002-6630-20171226-333

• Nutrition & Hygiene • Previous Articles     Next Articles

Effect of Methionine Restriction on Gut Redox Status, Inflammation and Microbiota in High-Fat Diet-Fed Mice

ZHANG Yuanhong, YANG Yuhui, WANG Yanan, ZHANG Jiahong, GUO Haitao, SHI Yonghui, LE Guowei*   

  1. The State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
  • Online:2019-05-15 Published:2019-05-28

Abstract: Purpose: To investigate the effect of dietary methionine restriction (MR) on gut redox status, inflammation and microbiota in high-fat diet-fed mice. Methods: Twenty-seven male C57BL/6 mice were randomly and equally divided into control group (C: 0.86% methionine and 4% lard oil), high-fat diet group (HM: 0.86% methionine and 20% lard oil), high-fat diet with MR group (LM: 0.17% methionine and 20% lard oil). Body mass was recorded weekly. After 12 weeks, the mice were killed to collect blood plasma, cecum, ileum and colon. Plasma total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), lipopolysaccharide (LPS), and LPS-binding protein (LBP) contents were examined. Some indicators of oxidative stress in ileum and colon were determined. The expression of tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) mRNA in ileum were determined by polymerase chain reaction (PCR). DNA was extracted from cecal contents for analysis of microbiota changes by terminal restriction fragment length polymorphism. DNA was extracted from colonic contents for analysis of microbiota changes by high throughput sequencing. Results: Compared with HM, LM significantly decreased body mass, plasma TC, TG and LDL-C, and colonic malondialdehyde (MDA) level (P < 0.05, P < 0.01). In contrast, LM significantly increased plasma HDL-C (P < 0.01), total antioxidant capacity (T-AOC) and glutathione/oxidized glutathione (GSH/GSSG) ratio in ileum (P < 0.05), and GSH/GSSG ratio and MDA level in colon (P < 0.05). Similarly, LM significantly decreased the plasma levels of LPS and LBP, the expression of TNF-α and IL-6 mRNA in ileum (P < 0.05), but significantly increased the Shannon-Weiner index and even index of cecal microbiota (P < 0.05) and the relative abundance of Bifidobacterium and Oscillospira in colonic contents (P < 0.05). Conclusion: MR can improve the intestinal redox status, inflammatory response and gut microbiota in high-fat diet-fed mice.

Key words: methionine restriction, high-fat diet, inflammation, intestinal redox status, gut microbiota

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