Effect of Chicoric Acid on Oxidative Lipid and DNA Damage Induced by Reactive Oxygen Species

doi:10.7506/spkx1002-6630-201813035

Abstract

Abstract: In this study, the effect of chicoric acid on oxidative lipid and DNA damage induced by reactive oxygen species was investigated using Cu2+/H2O2 and 2,2’-azobis(2-amidinopropane)dihydrochloride (AAPH) systems. Mouse liver and brain homogenate, herring sperm DNA and pBR322 DNA were selected as lipid and DNA damage model, respectively. The thiobarbituric acid method was used to evaluate the levels of lipid and herring sperm DNA oxidative damage. The degree of oxidative damage of pBR322 DNA was determined by agarose gel electrophoresis. Results showed that chicoric acid exhibited significant protective effects on lipid and DNA in Cu2+/H2O2 system in a certain concentration range. However, the protective effect of chicoric acid on lipid and DNA was abated at high concentrations. Chicoric acid at 100 μmol/L even showed prooxidant activity on pBR322 DNA. In AAPH system, chicoric acid protected lipid and DNA effectively within the experimental concentration range. In conclusion, chicoric acid has a pronounced protective effect on lipid and DNA, whereas chicoric acid at high concentrations aggravates oxidative lipid and DNA damage induced by hydroxyl free radicals.

Key words: chicoric acid, lipid, DNA, oxidative damage, Cu2+/H2O2, 2,2’-azobis(2-amidinopropane)dihydrochloride

CLC Number:

TS201.4

XIAO Haifang, FU Jingjing, WANG Youling, LI Fanyue, SONG Yuanda. Effect of Chicoric Acid on Oxidative Lipid and DNA Damage Induced by Reactive Oxygen Species[J]. FOOD SCIENCE, 2018, 39(13): 235-240.

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Effect of Chicoric Acid on Oxidative Lipid and DNA Damage Induced by Reactive Oxygen Species

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