Analysis of Partial DNA Sequence of CO I Gene and Its Application for Species Identification of Thirteen Species of Puffer Fish

doi:10.7506/spkx1002-6630-201824022

FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (24): 145-148.doi: 10.7506/spkx1002-6630-201824022

• Bioengineering • Previous Articles Next Articles

Analysis of Partial DNA Sequence of CO I Gene and Its Application for Species Identification of Thirteen Species of Puffer Fish

CHEN Wenbing1, MIAO Tingyu1, WENG Guozhu2, CHEN Rongbin3, SHAO Biying 1, PENG Juan1, JIANG Shuxun1

(1. Inspection and Quarantine Technology Center, Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350001, China;2. Putian Entry-Exit Inspection and Quarantine Bureau, Putian 351100, China;3. Fisheries College, Jimei University, Xiamen 361021, China)

Online:2018-12-25 Published:2018-12-17

Abstract

Abstract: Partial fragments of the cytochrome oxidase subunit I (CO I) genes from13 species in 3 genera of puffer fish and 9 samples from unknown species collected in Fujian province and Hainan province were amplified by polymerase chain reaction (PCR) using universal primers and the PCR products were sequenced. The DNA sequences of 13 known species were submitted to the GenBank with accession numbers. The target gene sequence was 681 bp in length for all species. Sequence alignment was carried out using DNAMAN V6 software, and a phylogenetic tree among species was established. Thirteen known species were divided into 4 groups, with 84% homology between the groups and 90%–100% within the groups. Nine samples of unknown puffer fish species were classed into 2 groups: the Takifugu and Gastrophysus genera; HNW2 was identified as Gastrophysus lunaris, HNW3, HNW4, FJW2, and FJW5 as G. spadiceus, HNW1 as G. gloveri and FJW1, FJW3 and FJW4 as T. oblongus.

Key words: puffer fish, CO I gene, DNA barcode, species identification

CLC Number:

S917.4
Q78

CHEN Wenbing, MIAO Tingyu, WENG Guozhu, CHEN Rongbin, SHAO Biying , PENG Juan, JIANG Shuxun. Analysis of Partial DNA Sequence of CO I Gene and Its Application for Species Identification of Thirteen Species of Puffer Fish[J]. FOOD SCIENCE, 2018, 39(24): 145-148.

[1]	HU Yiran, LI Jieqing, LIU Honggao, FAN Maopan, WANG Yuanzhong. Species Identification of Common Wild Edible Bolete in Yunnan by Fourier Transform Mid-infrared Spectroscopy Coupled with Support Vector Machine [J]. FOOD SCIENCE, 2021, 42(8): 248-256.
[2]	BAI Wenming, XING Ranran, CHEN Liping, PENG Tao, LEI Hongtao, CHEN Ying. DNA Barcoding for Identification of Toxic Amanita Species [J]. FOOD SCIENCE, 2021, 42(4): 278-286.
[3]	SHANG Ke, LIANG Hengxing, ZHANG Biao, DUAN Qingzi, WANG Wei, ZHANG Yu. Application of Real-Time PCR Assay in the Identification of Caprinae Meat [J]. FOOD SCIENCE, 2020, 41(12): 318-325.
[4]	YAO Sen, LIU Honggao, LI Tao, LI Jieqing, WANG Yuanzhong,. Data Fusion of Fourier Transform Infrared and UV Spectra for the Discrimination of Bolete Mushrooms [J]. FOOD SCIENCE, 2018, 39(20): 302-307.
[5]	CHEN Wenbing, SHAO Biying , MIAO Tingyu, PENG Juan, CHEN Bin, ZHANG Zhideng, JIANG Shuxun. Identification of Six Eel Species Using Polygenic DNA Barcoding [J]. FOOD SCIENCE, 2018, 39(2): 163-169.
[6]	XING Ranran, WU Yajun, CHEN Ying. DNA Metabarcoding in Food Species Identification: Current Applications and Future Prospects [J]. FOOD SCIENCE, 2018, 39(13): 280-288.
[7]	PAN Yanyi, QIU Deyi, CHEN Jian, YANG Weidong, YUE Qiaoyun. Mini-DNA Barcode for Identification of Multiple Animal-Derived Ingredients [J]. FOOD SCIENCE, 2018, 39(10): 326-332.
[8]	CHEN Wenbing, MIAO Tingyu, PENG Juan, SHAO Biying, CHEN Bin, JIANG Shuxun, ZHANG Zhideng. Identification of Anguilla rostrata, Anguilla anguilla and Anguilla japonica Using DNA Barcoding Based on 16S rRNA Gene [J]. FOOD SCIENCE, 2017, 38(4): 283-289.
[9]	LIU Rui, YU Zhanglong, XUE Chong, WU Yi, WU Xinyan, LI Mingyi. Identification of Bacterial Species and Microbial Inactivation by Acidic Electrolyzed Water on Commercial Bean Sprouts [J]. FOOD SCIENCE, 2017, 38(17): 168-173.
[10]	CHEN Wenbing, WENG Guozhu, CHEN Rongbin, MIAO Tingyu, SHAO Biying, JIANG Shuxun, PENG Juan. Partial DNA Sequence Analysis of 16S rRNA Gene in Puffer Fish [J]. FOOD SCIENCE, 2015, 36(21): 140-144.
[11]	QU Liang-miao1,2, CHEN Wen-bing1,3,*, MIAO Ting-yu1,3, SHAO Bi-ying1,3, PENG Juan1,3, JIANG Shu-xun1,3. Confirmation of PCR Results for Puffer Fish Components by Restriction Endonuclease Digestion [J]. FOOD SCIENCE, 2014, 35(8): 169-173.
[12]	GAO Jing, WEI Di, ZHANG Gui-rong, NIE Ling-yun. Recent Progress on Commonly Used Techniques for Identification of Meat Species [J]. FOOD SCIENCE, 2014, 35(11): 356-360.
[13]	YANG Ji-xia1,2，CHEN Zhi-lan3，YANG Hai-yan4，HUANG Ai-xiang5，CHEN Zong-dao1,2，HE Zhi-fei1,2,*. Identification and Characterization of Lactic Acid Bacteria from Chinese Yak Milk Cheeses [J]. FOOD SCIENCE, 2013, 34(9): 198-204.
[14]	LI Xin-guang，WANG Lu，ZHAO Feng，MA Li-ping，SUN Yong，ZHOU De-qing. Application of DNA Barcoding to Identify Commercial Fish and Fish Products [J]. FOOD SCIENCE, 2013, 34(18): 337-342.
[15]	LU Hong-fa1，ZHENG Shan-jian1，FANG Mei-juan1，PAN Li-xin1，CHEN Shu-fen2. Identification of Species and Nutritional Analysis of Alpine Thin-shelled Cipangopaludina from Qingyuan County, Zhejiang Province [J]. FOOD SCIENCE, 2013, 34(17): 309-312.

Analysis of Partial DNA Sequence of CO I Gene and Its Application for Species Identification of Thirteen Species of Puffer Fish

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments