Abstract: To study the reather strong antioxidation action,the components of black sogbean.Pigment and crudepolysaccharide were extracted from the seed of Glycine max(L.) Merr (black soybean),The inhibitory effects ofpigment and crude polysaccharide from black soybean on whole blood chemiluminescence and on active oxygen havebed studied.The results showed that the pigment black soybean and crude polysaccharide from black soybean canobviously inhibit chemiluminescence in whole blood and can also scavenge superoxide anion radicals hydroxylradicals, hydrogen peroxide with dose-effect relationship.A ccording to the value of antioxidation, the pigment fromblack soybean can scavenge directly the active oxygen generated by cellnlar or noncellular system and the cradepolysaccharide from black soybean has immunological inhibition effect on phagocyte.These results suggest that theblack soybean has the function of delaying aging,probably related to the direct or indirect scavenging of the activeoxygen produced by cellular or non-cellular systems.by the pigment and crude polysaccharide from black soybean.

Key words: Black soybean pigment, Polysaccharide, Chemiluminescence in whole Blood, Antioxidation, Active oxygen