FOOD SCIENCE ›› 2000, Vol. 21 ›› Issue (1): 9-10.

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 LI  Bao-Cai, WEN  Ke-Wei, SHENG  Guo-Ying, FU  Jia-Mo   

  • Online:2000-01-15 Published:2011-11-25

Abstract: The extracts from enzymolysis liquid of sheep placenta ware extracted by diethyl ether and then saponated in KOH-CH3CH2OH.extracted again by diethyl ether, and finally passed a mini-silica gel chromatography column. The elutions were derived by BSTFA and analysed on GC-MC.The results show at that the extracts from enzymolysis liquid of sheep placenta consisted of mainly mainly acids. cholesterol and a small amount of female hormones the C 16∶0.C 18∶0. and C 18∶1△9 acids were dominant in fatty acid series .The amount of cholesterol was highest both in sterol series and in extracts. The single ion m/z 416 and the characteristics of mass spectrum demonstrated that the estradiol existed in extracts .but in quite small amount.

Key words: Enzymolysis lipuid of sheep placenta, Extracts by diethylether, fatty acids, sterols cholesterol, hormone GC-MS analysis