Studies on the Fermentative Production of 2-Keto-D-gluconic Acid by Pseudomonas fluorescens in Fed-batch Culture

Abstract

Abstract: The effects of feeding glucose on the production of 2-keto-D-gluconic acid by Pseudomonas fluorescens AR4 werestudied, and the application of fed-batch culture in the industrial production was also investigated in a 50kL fermentor. The resultsshowed that the concentration of the product could be increased effectively in fed-batch culture, the suitable concentration ofresidual glucose in the fermentation broth was 3%～6% while beginning to feed glucose, and the culture method and the strainAR4 could be applied in the industrial fermentation process.

Key words: keto-D-gluconic acid, Pseudomonas fluorescens AR4, fed-batch culture

SUN Wen-Jing, ZHAO Feng-Mei, YANG Qing-Wen, GUO Jin-Quan, QIN Li, LIU Jing-Ze. Studies on the Fermentative Production of 2-Keto-D-gluconic Acid by Pseudomonas fluorescens in Fed-batch Culture[J]. FOOD SCIENCE, 2004, 25(11): 115-117.

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Studies on the Fermentative Production of 2-Keto-D-gluconic Acid by Pseudomonas fluorescens in Fed-batch Culture

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