In Vitro Study on HL-60 Cell Apoptosis Induced by (-)Epigalocatechin Galtate of Tea Polyphenols

Abstract

Abstract: Object: To evaluate the nature occurred new anti-leukemia candidate drug, apoptosis inducing effect of main ingredient(-)epigalocatechin galtate (EGCG) of tea polyphenols was studied in vitro. Methods: The apoptosis inducing effect of EGCG and theoptimal duration and concentration of its action were observed on the cultured human promyelocytic leukemia HL-60 cell line in vitrounder light and transmission electro-microscopy as well as DNA agarose gel electrophoresis. Result: The cultured HL-60 cells growthwas inhibited and DNA ladder was clearly shown on electrophoresis and the ultrastructure of the cells exhibited significant alterationsafter treated with EGCG. At the concentration of 250μg/ml for 6h showed the most evident apoptosis effect. Conclusion: Thetea ingredient EGCG did induce apoptosis of HL-60 cells in vitro, and might be a candidate of anti-leukemia remedy.

Key words: epigalocatechin galtate(EGCG), leukemia, cell apoptosis

REN Li-Li. In Vitro Study on HL-60 Cell Apoptosis Induced by (-)Epigalocatechin Galtate of Tea Polyphenols[J]. FOOD SCIENCE, 2004, 25(5): 174-177.

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In Vitro Study on HL-60 Cell Apoptosis Induced by (-)Epigalocatechin Galtate of Tea Polyphenols

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