Determination of Total Triterpene in Lagerstroemia specious L.by Thin Layer Chromatography-spectrophotometry

Abstract

Abstract: The procedure of thin layer chromatography-spectrophotometry analysis of total triterpene in Lagerstroemia specious L. leaf was investigated in this paper. The results showed that the accuracy of determination was high, processing with the following procedure: acetone (4:1 in volume ratio) as developer,0.3ml of 5% vanillin/glacial acetic acid added to 1ml perchloric acid solution, heating 15 minutes at 60℃, and the absorbance measured at 542 nm after being added 5ml glacial acetic acid and cooling. The recovery of the procedure was 100.22% and RSD 2.05%(n=5).The result showed no sharp difference with HPLC result.

Key words: Lagerstroemia specious L, total triterpene, thin layer chromatography, spectrophotometry

ZONG Wei, XIA Wen-Shui, CUI Bao-Liang. Determination of Total Triterpene in Lagerstroemia specious L.by Thin Layer Chromatography-spectrophotometry[J]. FOOD SCIENCE, 2005, 26(4): 222-225.

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Determination of Total Triterpene in Lagerstroemia specious L.by Thin Layer Chromatography-spectrophotometry

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