FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (7): 78-81.

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Study on Tracking Bacteriostatic Components in Puchiin

 LIU  Xin, HAO  Shu-Xian, ZHAO  Li-Chao, HUANG  Hui, CHEN  Yong-Quan   

  1. 1.College of Food South China Agricultural University;2.Nutrition and FoodEngineering Lab, South China Sea Fisheries Institute
  • Online:2005-07-15 Published:2011-09-19

Abstract: The biological development technique was used to provide a qualitative analysis method for tracking bacferiostaficcomponents in puchiin. In the study, the Bacillus subtilis was regarded as indicator organism. The optimum conditions wereobtained as follows with 200mg/ml acetone solution extract: the volumn of sample was 4μl, the diffusion time and the culturetime were 10min and 10h respectively under the temperature of 37℃. Six different antibacterial points appeared with themixture of chloroform and carbinol in the ratio of 8.5 to 1.5.

Key words: puchiin, bacteriostatic, bioautography, Rf