Separation and Identification of All E-lutein and E-zeaxanthin in Foods with C30-HPLC-PDA

Abstract

Abstract: By HPLC equipped with PDA, all E-lutein and E-zeaxanthin in foods were readily separated on a C30 column under following conditions: YMCTM Carotenoid S-5 column (4.6×250mm); mobile phase A= acetonitrile-methanol-spasmolytol(75: 25:0.05); mobile phase B=methyl tert-butyl ether-spasmolytol (100:0.05); linear gradient: B increased from 0 to 55% in 20 minutes at the flow rate of 1.0 ml/min; PDA wavelengths range: 260～700nm and sample injection volume: 20μl. According to their chromatographic behavior and spectral characteristics, two fractions, all E-lutein and E-zeaxanthin are all identified. These results form the base to quantify those two fractions with C30-HPLC-PDA.

Key words: lutein, zeaxathin, C30 column

HUI Bo-Di, Li-Jing, Pei-Ling-Peng. Separation and Identification of All E-lutein and E-zeaxanthin in Foods with C30-HPLC-PDA[J]. FOOD SCIENCE, 2006, 27(9): 151-154.

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Separation and Identification of All E-lutein and E-zeaxanthin in Foods with C30-HPLC-PDA

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