Abstract: Using the lotus leaves as raw material,after extracting alkaloids from lotus leaves by 30% ethanol, the effects of static adsorption and desorption of macroporous resin -PA(13-40), D101 and HP-20 for lotus alkloids were compared. Then on the basis of screening the best macroporous resin with higher adsorbing rate and with desorbing amount, the effects of the desorbing quantity,purity and yield rate of alkloids at different eluting velocities by the desorption gradient of different eluting density of ethanol with volume 3 BV were studied, and the effects of desorbing quantity, purity and yield rate of alkloids by the same gradient of different eluting density under the best eluting velocity were also studied. The results showed that D101 is the best among the three kinds of resin. To separate and purify lotus alkaloids by D101 at 1.5 BV/h with 3 BV desorbing volume of ethanol concentration 20%, 40%, 60%, 80% ethanol gradient desorption is proved to be the best, with the total quantity of eluted alkaloid, yield rate, the total purity reaching 10.8226 mg, 62.9%, 4.86% in sequence. When only collecting the desorbing liquid with concentration above 80% ethanol, the purity of lotus alkaloids can reach 32.56%, which is valuable to process higher purity lotus alkaloids.

Key words: lotus leaves, alkaloid, column separation and purification, static adsorption, dynamic adsorption